Defining totipotency using criteria of increasing stringency
Defining totipotency using criteria of increasing stringency
Abstract Totipotency is the ability of a single cell to give rise to all the differentiated cells that build the conceptus, yet how to capture this property in vitro remains incompletely understood. Defining totipotency relies upon a variety of assays of variable stringency. Here we describe criteria to define totipotency. We illustrate how distinct criteria of increasing stringency can be used to judge totipotency by evaluating candidate totipotent cell types in the mouse, including early blastomeres and expanded or extended pluripotent stem cells. Our data challenge the notion that expanded or extended pluripotent states harbor increased totipotent potential relative to conventional embryonic stem cells under in vivo conditions.
De Geest Natalie、Petropoulos Sophie、Schell John Paul、Murray Alexander、To San Kit、Jurisicova Andrea、Pasque Vincent、Rossant Janet、Posfai Eszter、El Bakkali Mouna、Lanner Fredrik、Bradshaw Brian、Rovic Isidora、Janiszewski Adrian、Pardon Tine、Kumar Pankaj、Talon Irene
KU Leuven-University of Leuven, Department of Development and RegenerationDepartment of Clinical Sciences, Intervention and Technology, Karolinska Institutet||Division of Obstetrics and Gynecology, Karolinska Universitetssjukhuset||Centre de recherche du centre hospitalier de l?ˉuniversit¨| de montr¨|al (CRCHUM), University of Montreal, Department of MedicineDepartment of Clinical Sciences, Intervention and Technology, Karolinska Institutet||Division of Obstetrics and Gynecology, Karolinska UniversitetssjukhusetProgram in Developmental and Stem Cell Biology, Hospital for Sick ChildrenKU Leuven-University of Leuven, Department of Development and RegenerationLunenfeld Tanenbaum Research Institute, Sinai Health System||Departments of Obstetrics and Gynecology and Department of PhysiologyKU Leuven-University of Leuven, Department of Development and RegenerationProgram in Developmental and Stem Cell Biology, Hospital for Sick ChildrenProgram in Developmental and Stem Cell Biology, Hospital for Sick Children||Department of Molecular Biology, Princeton UniversityKU Leuven-University of Leuven, Department of Development and RegenerationDepartment of Clinical Sciences, Intervention and Technology, Karolinska Institutet||Division of Obstetrics and Gynecology, Karolinska Universitetssjukhuset||Ming Wai Lau Center for Reparative Medicine, Stockholm node, Karolinska InstitutetProgram in Developmental and Stem Cell Biology, Hospital for Sick ChildrenLunenfeld Tanenbaum Research Institute, Sinai Health SystemKU Leuven-University of Leuven, Department of Development and RegenerationKU Leuven-University of Leuven, Department of Development and RegenerationDepartment of Clinical Sciences, Intervention and Technology, Karolinska Institutet||Division of Obstetrics and Gynecology, Karolinska UniversitetssjukhusetKU Leuven-University of Leuven, Department of Development and Regeneration
细胞生物学分子生物学遗传学
De Geest Natalie,Petropoulos Sophie,Schell John Paul,Murray Alexander,To San Kit,Jurisicova Andrea,Pasque Vincent,Rossant Janet,Posfai Eszter,El Bakkali Mouna,Lanner Fredrik,Bradshaw Brian,Rovic Isidora,Janiszewski Adrian,Pardon Tine,Kumar Pankaj,Talon Irene.Defining totipotency using criteria of increasing stringency[EB/OL].(2025-03-28)[2025-04-29].https://www.biorxiv.org/content/10.1101/2020.03.02.972893.点此复制
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