The identification of potent and selective antibodies for Serine/threonine-protein kinase TBK1, for use in immunoblot, immunofluorescence and immunoprecipitation
The identification of potent and selective antibodies for Serine/threonine-protein kinase TBK1, for use in immunoblot, immunofluorescence and immunoprecipitation
ABSTRACT TBK1 is a serine-threonine kinase that has been linked to a number of diseases, including amyotrophic lateral sclerosis and frontotemporal dementia. Reproducible research on TBK1 has been hampered by the lack of well characterized antibodies. In this study, we characterized 11 commercial antibodies for immunoblot, immunofluorescence and immunoprecipitation, using a knock-out cell line as the control. For each application, we identified several potent and selective antibodies that will facilitate studies on TBK1.
Alshafie Walaa、Edwards Aled M.、Durcan Thomas M、McPherson Peter S.、Fotouhi Maryam、Shlaifer Irina、Laflamme Carl
Tanenbaum Open Science Institute, Structural Genomics Consortium, Montreal Neurological Institute, McGill UniversityTanenbaum Open Science Institute, Structural Genomics Consortium, Montreal Neurological Institute, McGill UniversityEarly Drug Discovery Unit (EDDU), Montreal Neurological Institute, McGill UniversityTanenbaum Open Science Institute, Structural Genomics Consortium, Montreal Neurological Institute, McGill UniversityTanenbaum Open Science Institute, Structural Genomics Consortium, Montreal Neurological Institute, McGill UniversityEarly Drug Discovery Unit (EDDU), Montreal Neurological Institute, McGill UniversityTanenbaum Open Science Institute, Structural Genomics Consortium, Montreal Neurological Institute, McGill University
基础医学生物科学研究方法、生物科学研究技术分子生物学
Alshafie Walaa,Edwards Aled M.,Durcan Thomas M,McPherson Peter S.,Fotouhi Maryam,Shlaifer Irina,Laflamme Carl.The identification of potent and selective antibodies for Serine/threonine-protein kinase TBK1, for use in immunoblot, immunofluorescence and immunoprecipitation[EB/OL].(2025-03-28)[2025-07-02].https://www.biorxiv.org/content/10.1101/2022.06.03.494699.点此复制
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