Whole Genome Bisulphite Sequencing Using the Illumina Hiseq X System
Whole Genome Bisulphite Sequencing Using the Illumina Hiseq X System
Abstract The Illumina HiSeq X platform has helped to reduce the cost of whole genome sequencing substantially, but its application for bisulphite sequencing is not straightforward. We describe the optimization of a library preparation and sequencing approach that maximizes the yield and quality of sequencing, and the elimination of a previously unrecognized artefact affecting several percent of bisulphite sequencing reads.
Suzuki M.、Wos F.、DeGrazia J.、Ishii J.、Bloom T.、Germer S.、Liao W.、Greally J.M.、Zody M.C.、Johnston A.D.
Center for Epigenomics and Department of Genetics, Albert Einstein College of MedicineNew York Genome CenterNew York Genome CenterNew York Genome CenterNew York Genome CenterNew York Genome CenterNew York Genome CenterCenter for Epigenomics and Department of Genetics, Albert Einstein College of MedicineNew York Genome CenterCenter for Epigenomics and Department of Genetics, Albert Einstein College of Medicine
生物科学研究方法、生物科学研究技术遗传学分子生物学
Suzuki M.,Wos F.,DeGrazia J.,Ishii J.,Bloom T.,Germer S.,Liao W.,Greally J.M.,Zody M.C.,Johnston A.D..Whole Genome Bisulphite Sequencing Using the Illumina Hiseq X System[EB/OL].(2025-03-28)[2025-04-27].https://www.biorxiv.org/content/10.1101/193193.点此复制
评论