Intestinal fungal dynamics and linkage to hematopoietic cell transplantation outcomes
Intestinal fungal dynamics and linkage to hematopoietic cell transplantation outcomes
Abstract Allogeneic hematopoietic cell transplantation (allo-HCT) induces profound shifts in the intestinal bacterial microbiota. The dynamics of intestinal fungi and their impact on clinical outcomes have not yet been integrated into a model of microbiota function during allo-HCT. Here, we combined parallel high-throughput fungal ITS1 amplicon sequencing, bacterial 16S amplicon sequencing, and fungal cultures to reveal striking trans-kingdom dynamics and their association with patient outcomes. We saw that the overall density and the biodiversity of intestinal fungi were stable during allo-HCT, but the species composition changed drastically from day to day. We identified a subset of patients with fungal dysbiosis characterized by culture positivity, stable expansion of Candida parapsilosis complex species, and distinct trans-kingdom microbiota profiles. These patients had worse overall survival and higher transplant-related mortality independent of candidemia. Our data expand the clinical significance of the mycobiota and suggest that targeting fungal dysbiosis may help to improve long-term patient survival.
Zhai Bing、Tosini Nicholas、Yasuma-Mitobe Keiko、Fontana Emily、Amoretti Luigi A、Perales Miguel A、Xavier Joao B、Taur Ying、Wright Roberta J、van den Brink Marcel R M、Hohl Tobias M、Ponce Doris M、Markey Kate A、Gjonbalaj Mergim、Rolling Thierry、Peled Jonathan U
Infectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center||Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer CenterInfectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center||Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer CenterInfectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center||Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer CenterInfectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer CenterInfectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer CenterAdult Bone Marrow Transplantation Service, Department of Medicine, Memorial Sloan Kettering Cancer Center||Department of Medicine, Weill Cornell Medical CollegeComputational and Systems Biology Program, Memorial Sloan Kettering Cancer CenterInfectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center||Department of Medicine, Weill Cornell Medical CollegeInfectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer CenterImmunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center||Adult Bone Marrow Transplantation Service, Department of Medicine, Memorial Sloan Kettering Cancer Center||Department of Medicine, Weill Cornell Medical CollegeInfectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center||Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center||Department of Medicine, Weill Cornell Medical CollegeAdult Bone Marrow Transplantation Service, Department of Medicine, Memorial Sloan Kettering Cancer Center||Department of Medicine, Weill Cornell Medical CollegeAdult Bone Marrow Transplantation Service, Department of Medicine, Memorial Sloan Kettering Cancer Center||Department of Medicine, Weill Cornell Medical CollegeInfectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center||Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer CenterInfectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center||Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center||Division of Infectious Diseases, First Department of Medicine, University Medical Center Hamburg-EppendorfAdult Bone Marrow Transplantation Service, Department of Medicine, Memorial Sloan Kettering Cancer Center||Department of Medicine, Weill Cornell Medical College
医药卫生理论医学研究方法微生物学
Zhai Bing,Tosini Nicholas,Yasuma-Mitobe Keiko,Fontana Emily,Amoretti Luigi A,Perales Miguel A,Xavier Joao B,Taur Ying,Wright Roberta J,van den Brink Marcel R M,Hohl Tobias M,Ponce Doris M,Markey Kate A,Gjonbalaj Mergim,Rolling Thierry,Peled Jonathan U.Intestinal fungal dynamics and linkage to hematopoietic cell transplantation outcomes[EB/OL].(2025-03-28)[2025-05-12].https://www.medrxiv.org/content/10.1101/2021.07.20.21260859.点此复制
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