Polymer skulls with integrated transparent electrode arrays for cortex-wide opto-electrophysiological recordings
Polymer skulls with integrated transparent electrode arrays for cortex-wide opto-electrophysiological recordings
ABSTRACT Electrophysiological and optical imaging provide complementary neural sensing capabilities – electrophysiological recordings have the highest temporal resolution, while optical imaging allows recording the activities of genetically defined populations at high spatial resolution. Combining these complementary, yet orthogonal modalities to perform simultaneous large-scale, multimodal sensing of neural activity across multiple brain regions would be very powerful. Here we show that transparent, inkjet-printed electrocorticography (ECoG) electrode arrays can be seamlessly integrated with morphologically conformant transparent polymer skulls for multimodal recordings across the cortex. These ‘eSee-Shells’ were implanted on transgenic mice expressing the Ca2+ indicator GCaMP6f in cortical excitatory cells and provided a robust opto-electrophysiological interface for over 100 days. eSee-Shells enable simultaneous mesoscale Ca2+ imaging and ECoG acquisition under anesthesia as well as in awake animals presented with sensory stimuli. eSee-Shells further show sufficient clarity and transparency to observe single-cell Ca2+ signals directly below the electrodes and interconnects. Simultaneous multimodal measurement of cortical dynamics reveals changes in both ECoG and Ca2+ signals that depend on the behavioral state.
Ghanbari Leila、Navabi Zahra S.、Carter Russell E.、Swisher Sarah L.、Kodandaramaiah Suhasa B.、Ebner Timothy J.、Fausner Skylar M. L.、Donaldson Preston D.
Department of Mechanical Engineering, University of Minnesota Twin CitiesDepartment of Mechanical Engineering, University of Minnesota Twin CitiesDepartment of Neuroscience, University of Minnesota, Twin CitiesDepartment of Electrical and Computer Engineering, University of Minnesota Twin CitiesDepartment of Mechanical Engineering, University of Minnesota Twin Cities||Department of Neuroscience, University of Minnesota, Twin Cities||Department of Biomedical Engineering, University of Minnesota Twin CitiesDepartment of Neuroscience, University of Minnesota, Twin CitiesDepartment of Mechanical Engineering, University of Minnesota Twin CitiesDepartment of Electrical and Computer Engineering, University of Minnesota Twin Cities
生物科学现状、生物科学发展生物科学研究方法、生物科学研究技术生物物理学生物工程学
Ghanbari Leila,Navabi Zahra S.,Carter Russell E.,Swisher Sarah L.,Kodandaramaiah Suhasa B.,Ebner Timothy J.,Fausner Skylar M. L.,Donaldson Preston D..Polymer skulls with integrated transparent electrode arrays for cortex-wide opto-electrophysiological recordings[EB/OL].(2025-03-28)[2025-05-24].https://www.biorxiv.org/content/10.1101/2021.11.13.468490.点此复制
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