Evolutionary history of the global emergence of the Escherichia coli epidemic clone ST131
Evolutionary history of the global emergence of the Escherichia coli epidemic clone ST131
ABSTRACT BackgroundEscherichia coli sequence type 131 (ST131) has emerged globally as the most predominant lineage within this clinically important species, and its association with fluoroquinolone and extended-spectrum cephalosporin resistance impacts significantly on treatment. The evolutionary histories of this lineage, and of important antimicrobial resistance elements within it, remain unclearly defined. ResultsThis study of the largest worldwide collection (n = 215) of sequenced ST131 E. coli isolates to date demonstrates that clonal expansion of two previously recognized antimicrobial-resistant clades, C1/H30R and C2/H30Rx, started around 25 years ago, consistent with the widespread introduction of fluoroquinolones and extended-spectrum cephalosporins in clinical medicine. These two clades appear to have emerged in the United States, with the expansion of the C2/H30Rx clade driven by the acquisition of a blaCTX-M-15-containing IncFII-like plasmid that has subsequently undergone extensive rearrangement. Several other evolutionary processes influencing the trajectory of this drug-resistant lineage are described, including sporadic acquisitions of CTX-M resistance plasmids, and chromosomal integration of blaCTX-M within sub-clusters followed by vertical evolution. These processes are also occurring for another family of CTX-M gene variants more recently observed amongst ST131, the blaCTX-M-14/14-like group. ConclusionsThe complexity of the evolutionary history of ST131 has important implications for antimicrobial resistance surveillance, epidemiological analysis, and control of emerging clinical lineages of E. coli. These data also highlight the global imperative to reduce specific antibiotic selection pressures, and demonstrate the important and varied roles played by plasmids and other mobile genetic elements in the perpetuation of antimicrobial resistance within lineages.
Kasarskis Andrew、Price Lance B.、Modernising Medical Microbiology Informatics Group (MMMIG)、Turner Paul、Anson Luke W.、Batty Elizabeth M.、Wilson Daniel J.、Phetsouvanh Rattanaphone、Johnson Timothy J.、Johnson James R.、Didelot Xavier、Sheppard Anna E.、Kos Veronica、de Maio Nicola、Moore Catrin E.、Peto Timothy E. A.、Stoesser Nicole、Pankhurst Louise、Manges Amee R.、Sebra Robert、Walker A. Sarah、Crook Derrick W.、Sokurenko Evgeni、Wyllie David
Icahn Institute and Department of Genetics and Genomic Sciences, Icahn School of MedicineTranslational Genomics Research Institute (TGen) NorthModernizing Medical Microbiology Consortium, Nuffield Department of Clinical MedicineCambodia-Oxford Medical Research Unit, Angkor Hospital for ChildrenModernizing Medical Microbiology Consortium, Nuffield Department of Clinical MedicineInfection Innovative Medicines Unit, AstraZeneca R&D Boston, WalthamModernizing Medical Microbiology Consortium, Nuffield Department of Clinical MedicineLao-Oxford-Mahosot Hospital Wellcome Trust Research Unit, Mahosot Hospital, VientianeCollege of Veterinary Medicine, University of MinnesotaMinneapolis Veterans Affairs Health Care System||Department of Medicine, University of MinnesotaDepartment of Infectious Disease Epidemiology, School of Public HealthModernizing Medical Microbiology Consortium, Nuffield Department of Clinical MedicineInfection Innovative Medicines Unit, AstraZeneca R&D Boston, WalthamModernizing Medical Microbiology Consortium, Nuffield Department of Clinical MedicineCambodia-Oxford Medical Research Unit, Angkor Hospital for ChildrenModernizing Medical Microbiology Consortium, Nuffield Department of Clinical MedicineModernizing Medical Microbiology Consortium, Nuffield Department of Clinical MedicineModernizing Medical Microbiology Consortium, Nuffield Department of Clinical MedicineUniversity of British Columbia, School of Population and Public HealthIcahn Institute and Department of Genetics and Genomic Sciences, Icahn School of MedicineModernizing Medical Microbiology Consortium, Nuffield Department of Clinical MedicineModernizing Medical Microbiology Consortium, Nuffield Department of Clinical MedicineDepartment of Microbiology, University of WashingtonModernizing Medical Microbiology Consortium, Nuffield Department of Clinical Medicine
医药卫生理论医学研究方法微生物学
Kasarskis Andrew,Price Lance B.,Modernising Medical Microbiology Informatics Group (MMMIG),Turner Paul,Anson Luke W.,Batty Elizabeth M.,Wilson Daniel J.,Phetsouvanh Rattanaphone,Johnson Timothy J.,Johnson James R.,Didelot Xavier,Sheppard Anna E.,Kos Veronica,de Maio Nicola,Moore Catrin E.,Peto Timothy E. A.,Stoesser Nicole,Pankhurst Louise,Manges Amee R.,Sebra Robert,Walker A. Sarah,Crook Derrick W.,Sokurenko Evgeni,Wyllie David.Evolutionary history of the global emergence of the Escherichia coli epidemic clone ST131[EB/OL].(2025-03-28)[2025-06-23].https://www.biorxiv.org/content/10.1101/030668.点此复制
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