Plant Scaffolds Support Motor Recovery and Regeneration in Rat Spinal Cord Injury
Plant Scaffolds Support Motor Recovery and Regeneration in Rat Spinal Cord Injury
Abstract As of yet, no standard of care incorporates the use of a biomaterial to treat traumatic spinal cord injury (SCI)1–5. However, intense development of biomaterials for treating SCI have focused on the fabrication of microscale channels to support the regrowth of axons while minimizing scar tissue formation6–10. We previously demonstrated that plant tissues can be decellularized and processed to form sterile, biocompatible and implantable biomaterials that support cell infiltration and vascularization in vivo11–13 Notably, the vascular bundles of plant tissues are also composed of microscale channels with geometries thought to be relevant for supporting neural tissue regeneration14,15. We hypothesized that decellularized vascular bundles would support neural regeneration and the recovery of motor function. Therefore, rats which received a completeT8-T9 spinal cord transection were implanted with plant-derived channeled scaffolds. Animals which received the scaffolds alone, with no therapeutic stem cells or other interventions, demonstrated a significant and stable improvement in motor function over six months compared to controls. Histological analysis reveals minimal scarring and axonal regrowth through the scaffolds, further confirmed with tracer studies. Taken together, our work defines a novel route for exploiting naturally occurring plant microarchitectures to support the repair of functional spinal cord tissue.
Shore Isabel、Galuta Ahmad、Walker Krystal L. A.、Modulevsky Daniel J.、Tsai Eve C.、Pelling Andrew E.、Cuerrier Charles M.、Obhi Ras-Jeevan K.、Hickey Ryan J.、Leblanc-Latour Maxime
Department of Physics, STEM Complex, 150 Louis Pasteur Pvt., University of Ottawa||Spiderwort Inc.Program in Neuroscience, Ottawa Hospital Research InstituteProgram in Neuroscience, Ottawa Hospital Research InstituteDepartment of Physics, STEM Complex, 150 Louis Pasteur Pvt., University of Ottawa||Department of Biology, Gendron Hall, 30 Marie Curie, University of OttawaProgram in Neuroscience, Ottawa Hospital Research Institute||Division of Neurosurgery, Department of Surgery, The Ottawa Hospital||Department of Cellular and Molecular Medicine, Faculty of Medicine, University of OttawaDepartment of Physics, STEM Complex, 150 Louis Pasteur Pvt., University of Ottawa||Department of Biology, Gendron Hall, 30 Marie Curie, University of Ottawa||Spiderwort Inc.||Institute for Science Society and Policy, Simard Hall, 60 University, University of Ottawa||SymbioticA, School of Human Sciences, University of Western AustraliaDepartment of Physics, STEM Complex, 150 Louis Pasteur Pvt., University of Ottawa||Department of Biology, Gendron Hall, 30 Marie Curie, University of Ottawa||Spiderwort Inc.Department of Physics, STEM Complex, 150 Louis Pasteur Pvt., University of OttawaDepartment of Physics, STEM Complex, 150 Louis Pasteur Pvt., University of OttawaDepartment of Physics, STEM Complex, 150 Louis Pasteur Pvt., University of Ottawa||Spiderwort Inc.
神经病学、精神病学基础医学生物科学研究方法、生物科学研究技术
Shore Isabel,Galuta Ahmad,Walker Krystal L. A.,Modulevsky Daniel J.,Tsai Eve C.,Pelling Andrew E.,Cuerrier Charles M.,Obhi Ras-Jeevan K.,Hickey Ryan J.,Leblanc-Latour Maxime.Plant Scaffolds Support Motor Recovery and Regeneration in Rat Spinal Cord Injury[EB/OL].(2025-03-28)[2025-05-01].https://www.biorxiv.org/content/10.1101/2020.10.21.347807.点此复制
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