Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages
Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages
Abstract RNA viruses have short generation times and high mutation rates, allowing them to undergo rapid molecular evolution during epidemics. However, the extent of RNA virus phenotypic evolution within epidemics and the resulting effects on fitness and virulence remain mostly unknown. Here, we screened the 2015-2016 Zika epidemic in the Americas for lineage-specific fitness differences. We engineered a library of recombinant viruses representing twelve major Zika virus lineages and used them to measure replicative fitness within disease-relevant human primary cells and live mosquitoes. We found that two of these lineages conferred significant in vitro replicative fitness changes among human primary cells, but we did not find fitness changes in Aedes aegypti mosquitoes. Additionally, we found evidence for elevated levels of positive selection among five amino acid sites that define major Zika virus lineages. While our work suggests that Zika virus may have acquired several phenotypic changes during a short time scale, these changes were relatively moderate and do not appear to have enhanced virulence or transmission during the epidemic.
Oliveira Glenn、Saraf Sharada、Klitting Raphaelle、Weger-Lucarelli James、Leon Karla P.、Zolfaghari Ashley、Andersen Kristian G.、Grubaugh Nathan D.、Wohl Shirlee、Tsetsarkin Konstantin A.、Agarwal Rimjhim、Ontiveros Carlos O.、Harris Eva、Ebel Gregory D.、Vogels Chantal B.F.
Department of Immunology and Microbiology, The Scripps Research InstituteDepartment of Immunology and Microbiology, The Scripps Research InstituteDepartment of Immunology and Microbiology, The Scripps Research InstituteDepartment of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia TechDepartment of Immunology and Microbiology, The Scripps Research InstituteDepartment of Immunology and Microbiology, The Scripps Research InstituteDepartment of Immunology and Microbiology, The Scripps Research InstituteDepartment of Epidemiology of Microbial Diseases, Yale School of Public HealthDepartment of Immunology and Microbiology, The Scripps Research InstituteLaboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of HealthDepartment of Immunology and Microbiology, The Scripps Research InstituteDepartment of Immunology and Microbiology, The Scripps Research InstituteDivision of Infectious Diseases and Vaccinology, School of Public Health, University of CaliforniaDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State UniversityDepartment of Epidemiology of Microbial Diseases, Yale School of Public Health
基础医学分子生物学微生物学
Oliveira Glenn,Saraf Sharada,Klitting Raphaelle,Weger-Lucarelli James,Leon Karla P.,Zolfaghari Ashley,Andersen Kristian G.,Grubaugh Nathan D.,Wohl Shirlee,Tsetsarkin Konstantin A.,Agarwal Rimjhim,Ontiveros Carlos O.,Harris Eva,Ebel Gregory D.,Vogels Chantal B.F..Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages[EB/OL].(2025-03-28)[2025-05-23].https://www.biorxiv.org/content/10.1101/2022.02.01.478673.点此复制
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