Oxygen depletion and nitric oxide stimulate type IV MSHA pilus retraction in Vibrio cholerae via activation of the phosphodiesterase CdpA
Oxygen depletion and nitric oxide stimulate type IV MSHA pilus retraction in Vibrio cholerae via activation of the phosphodiesterase CdpA
ABSTRACT Bacteria use surface appendages called type IV pili to perform diverse activities including DNA uptake, twitching motility, and attachment to surfaces. Dynamic extension and retraction of pili is often required for these activities, but the stimuli that regulate these dynamics remain poorly characterized. To study this question, we use the bacterial pathogen Vibrio cholerae, which uses mannose-sensitive hemagglutinin (MSHA) pili to attach to surfaces in aquatic environments as the first step in biofilm formation. Here, we find that V. cholerae cells retract MSHA pili and detach from a surface in microaerobic conditions. This response is dependent on the phosphodiesterase CdpA, which decreases intracellular levels of cyclic-di-GMP (c-di-GMP) under microaerobic conditions to induce MSHA pilus retraction. CdpA contains a putative NO-sensing NosP domain, and we demonstrate that nitric oxide (NO) is necessary and sufficient to stimulate CdpA-dependent detachment. Thus, we hypothesize that microaerobic conditions result in endogenous production of NO (or an NO-like molecule) in V. cholerae. Together, these results describe a regulatory pathway that allows V. cholerae to rapidly abort biofilm formation. More broadly, these results show how environmental cues can be integrated into the complex regulatory pathways that control pilus dynamic activity and attachment in bacterial species.
Hossain Sajjad、Yu Yuanchen、Dalia Triana N.、Podicheti Ram C.、Jacobson Stephen C.、Brun Yves V.、McKinlay James B.、Hughes Hannah Q.、Rusch Douglas B.、Yildiz Fitnat H.、Dalia Ankur B.、Kappler Michael P.、Kysela David T.、Floyd Kyle A.、Boon Elizabeth M.、Anantharaman Sweta
Department of Chemistry and Institute of Chemical Biology & Drug Discovery, Stony Brook UniversityDepartment of Chemistry, Indiana UniversityDepartment of Biology, Indiana UniversityCenter for Genomics and Bioinformatics, Indiana UniversityDepartment of Chemistry, Indiana UniversityDepartment of Microbiology, Infectious Diseases, and Immunology, Universit¨| de Montr¨|alDepartment of Biology, Indiana UniversityDepartment of Biology, Indiana UniversityCenter for Genomics and Bioinformatics, Indiana UniversityDepartment of Microbiology and Environmental Toxicology, University of California ¨C Santa CruzDepartment of Biology, Indiana UniversityDepartment of Chemistry, Indiana UniversityDepartment of Microbiology, Infectious Diseases, and Immunology, Universit¨| de Montr¨|alDepartment of Microbiology and Environmental Toxicology, University of California ¨C Santa CruzDepartment of Chemistry and Institute of Chemical Biology & Drug Discovery, Stony Brook UniversityDepartment of Chemistry and Institute of Chemical Biology & Drug Discovery, Stony Brook University
微生物学分子生物学环境生物学
Hossain Sajjad,Yu Yuanchen,Dalia Triana N.,Podicheti Ram C.,Jacobson Stephen C.,Brun Yves V.,McKinlay James B.,Hughes Hannah Q.,Rusch Douglas B.,Yildiz Fitnat H.,Dalia Ankur B.,Kappler Michael P.,Kysela David T.,Floyd Kyle A.,Boon Elizabeth M.,Anantharaman Sweta.Oxygen depletion and nitric oxide stimulate type IV MSHA pilus retraction in Vibrio cholerae via activation of the phosphodiesterase CdpA[EB/OL].(2025-03-28)[2025-05-29].https://www.biorxiv.org/content/10.1101/2021.04.20.440694.点此复制
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