Nuclear Pore Complex Acetylation Regulates mRNA Export and Cell Cycle Commitment in Budding Yeast
Nuclear Pore Complex Acetylation Regulates mRNA Export and Cell Cycle Commitment in Budding Yeast
Abstract Nuclear pore complexes (NPCs) mediate communication between the nucleus and the cytoplasm and regulate gene expression by interacting with transcription and mRNA export factors. We find that Esa1, the catalytic subunit of the yeast lysine acetyl-transferase NuA4 complex, acetylates the nuclear basket component Nup60 to promote mRNA export. Acetylation of Nup60 recruits mRNA export factors to the nuclear basket, including the scaffolding subunit of the Transcription and Export 2 (TREX-2) complex, Sac3. Esa1-dependent nuclear export of mRNAs promotes entry into S phase, and is inhibited by the Hos3 deacetylase in G1 daughter cells to restrain their premature commitment to a new cell division cycle. This mechanism also inhibits expression of the nutrient-regulated GAL1 gene specifically in daughter cells. These results reveal how acetylation contributes to the functional plasticity of NPCs in specific cell types, and demonstrate how the evolutionarily conserved NuA4 complex regulates gene expression dually at the level of mRNA synthesis and export by modifying the nucleoplasmic entrance to nuclear pores.
Gomar-Alba Merc¨¨、Pozharskaia Vasilisa、Kumar Arun、Charvin Gilles、Schaal Celia、Igual J. Carlos、Jacquel Basile、Mendoza Manuel
Institut de G¨|n¨|tique et de Biologie Mol¨|culaire et Cellulaire||Institut de Biotecnologia i Biomedicina (BIOTECMED) and Departament de Bioqu¨amica i Biologia Molecular, Universitat de Val¨¨nciaInstitut de G¨|n¨|tique et de Biologie Mol¨|culaire et CellulaireDepartment of Cell Biology, Universitat Pompeu Fabra (UPF)Institut de G¨|n¨|tique et de Biologie Mol¨|culaire et Cellulaire||Centre National de la Recherche Scientifique||Institut National de la Sant¨| et de la Recherche M¨|dicale||Universit¨| de StrasbourgInstitut de G¨|n¨|tique et de Biologie Mol¨|culaire et CellulaireInstitut de Biotecnologia i Biomedicina (BIOTECMED) and Departament de Bioqu¨amica i Biologia Molecular, Universitat de Val¨¨nciaInstitut de G¨|n¨|tique et de Biologie Mol¨|culaire et CellulaireInstitut de G¨|n¨|tique et de Biologie Mol¨|culaire et Cellulaire||Centre National de la Recherche Scientifique||Institut National de la Sant¨| et de la Recherche M¨|dicale||Universit¨| de Strasbourg
细胞生物学分子生物学生物科学理论、生物科学方法
Gomar-Alba Merc¨¨,Pozharskaia Vasilisa,Kumar Arun,Charvin Gilles,Schaal Celia,Igual J. Carlos,Jacquel Basile,Mendoza Manuel.Nuclear Pore Complex Acetylation Regulates mRNA Export and Cell Cycle Commitment in Budding Yeast[EB/OL].(2025-03-28)[2025-05-11].https://www.biorxiv.org/content/10.1101/2021.09.01.458533.点此复制
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