Analysis of diverse eukaryotes suggests the existence of an ancestral mitochondrial apparatus derived from the bacterial type II secretion system
Analysis of diverse eukaryotes suggests the existence of an ancestral mitochondrial apparatus derived from the bacterial type II secretion system
Abstract The type 2 secretion system (T2SS) is present in some Gram-negative eubacteria and used to secrete proteins across the outer membrane. Here we report that certain representative heteroloboseans, jakobids, malawimonads and hemimastigotes unexpectedly possess homologues of core T2SS components. We show that at least some of them are present in mitochondria, and their behaviour in biochemical assays is consistent with the presence of a mitochondrial T2SS-derived system (miT2SS). We additionally identified 23 protein families co-occurring with miT2SS in eukaryotes. Seven of these proteins could be directly linked to the core miT2SS by functional data and/or sequence features, whereas others may represent different parts of a broader functional pathway, possibly also involving the peroxisome. Its distribution in eukaryotes and phylogenetic evidence together indicate that the miT2SS-centred pathway is an ancestral eukaryotic trait. Our findings thus have direct implications for the functional properties of the early mitochondrion.
P¨￠nek Tom¨￠?、Dole?al Pavel、Horv¨￠thov¨￠ Lenka、Derelle Romain、Voleman Lubo?、Klime? Vladim¨ar、Petr? Mark¨|ta、Hryz¨￠kov¨￠ Kl¨￠ra、Gray Michael W.、Vinopalov¨￠ Martina、Vaitov¨￠ Zuzana、Kl¨￠p?t?ˉov¨￠ Veronika、Chami Mohamed、Guilvout Ingrid、Eli¨￠? Marek、Moty?kov¨￠ Al?b¨§ta、?epi?ka Ivan、Lang B. Franz、Francetic Olivera、Vl?ek ?estm¨ar、Harant Karel、?¨￠rsky Vojt¨§ch、Pyrih Jan、Markov¨￠ Lenka、Tsaousis Anastasios D.
Department of Biology and Ecology, Faculty of Science, University of OstravaDepartment of Parasitology, Faculty of Science, Charles UniversityDepartment of Parasitology, Faculty of Science, Charles UniversitySchool of Biosciences, University of BirminghamDepartment of Parasitology, Faculty of Science, Charles UniversityDepartment of Biology and Ecology, Faculty of Science, University of OstravaDepartment of Parasitology, Faculty of Science, Charles UniversityDepartment of Genetics and Microbiology, Faculty of Science, Charles UniversityDepartment of Biochemistry and Molecular Biology and Centre for Comparative Genomics and Evolutionary Bioinformatics, Dalhousie UniversityDepartment of Parasitology, Faculty of Science, Charles UniversityDepartment of Parasitology, Faculty of Science, Charles UniversityDepartment of Parasitology, Faculty of Science, Charles UniversityCenter for Cellular Imaging and NanoAnalytics, University of BaselInstitut Pasteur, Biochemistry of Macromolecular Interactions Unit, Department of Structural Biology and ChemistryDepartment of Biology and Ecology, Faculty of Science, University of OstravaDepartment of Parasitology, Faculty of Science, Charles UniversityDepartment of Zoology, Faculty of Science, Charles UniversityRobert Cedergren Centre for Bioinformatics and Genomics, D¨|partement de Biochimie, Universit¨| de Montr¨|alInstitut Pasteur, Biochemistry of Macromolecular Interactions Unit, Department of Structural Biology and ChemistryInstitute of Molecular Genetics, Czech Academy of SciencesProteomic core facility, Faculty of Science, Charles UniversityDepartment of Parasitology, Faculty of Science, Charles UniversityLaboratory of Molecular & Evolutionary Parasitology, RAPID group, School of Biosciences, University of KentDepartment of Parasitology, Faculty of Science, Charles UniversityLaboratory of Molecular & Evolutionary Parasitology, RAPID group, School of Biosciences, University of Kent
分子生物学细胞生物学微生物学
P¨￠nek Tom¨￠?,Dole?al Pavel,Horv¨￠thov¨￠ Lenka,Derelle Romain,Voleman Lubo?,Klime? Vladim¨ar,Petr? Mark¨|ta,Hryz¨￠kov¨￠ Kl¨￠ra,Gray Michael W.,Vinopalov¨￠ Martina,Vaitov¨￠ Zuzana,Kl¨￠p?t?ˉov¨￠ Veronika,Chami Mohamed,Guilvout Ingrid,Eli¨￠? Marek,Moty?kov¨￠ Al?b¨§ta,?epi?ka Ivan,Lang B. Franz,Francetic Olivera,Vl?ek ?estm¨ar,Harant Karel,?¨￠rsky Vojt¨§ch,Pyrih Jan,Markov¨￠ Lenka,Tsaousis Anastasios D..Analysis of diverse eukaryotes suggests the existence of an ancestral mitochondrial apparatus derived from the bacterial type II secretion system[EB/OL].(2025-03-28)[2025-04-29].https://www.biorxiv.org/content/10.1101/790865.点此复制
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