Telomere-to-telomere genome assembly of Phaeodactylum tricornutum
Telomere-to-telomere genome assembly of Phaeodactylum tricornutum
ABSTRACT Phaeodactylum tricornutum is a marine diatom with a growing genetic toolbox available and is being used in many synthetic biology applications. While most of the genome has been assembled, the currently available genome assembly is not a completed telomere-to-telomere assembly. Here, we used Oxford Nanopore long reads to build a telomere-to-telomere genome for Phaeodactylum tricornutum. We developed a graph-based approach to extract all unique telomeres, and used this information to manually correct assembly errors. In total, we found 25 nuclear chromosomes that comprise all previously assembled fragments, in addition to the chloroplast and mitochondrial genomes. We found that chromosome 19 has filtered long-read coverage and a quality estimate that suggests significantly less haplotype sequence variation than the other chromosomes. This work improves upon the previous genome assembly and provides new opportunities for genetic engineering of this species, including creating designer synthetic chromosomes.
Giguere Daniel J.、Bahcheli Alexander T.、Flatley Martin、Edgell David R.、Gloor Gregory B.、Karas Bogumil J.、Patel Rushali R.、Slattery Samuel S.
Department of Biochemistry, Schulich School of Medicine and Dentistry, Western UniversityDepartment of Biochemistry, Schulich School of Medicine and Dentistry, Western UniversitySuncor EnergyDepartment of Biochemistry, Schulich School of Medicine and Dentistry, Western UniversityDepartment of Biochemistry, Schulich School of Medicine and Dentistry, Western UniversityDepartment of Biochemistry, Schulich School of Medicine and Dentistry, Western UniversityDepartment of Biochemistry, Schulich School of Medicine and Dentistry, Western UniversityDepartment of Biochemistry, Schulich School of Medicine and Dentistry, Western University
遗传学分子生物学生物工程学
Giguere Daniel J.,Bahcheli Alexander T.,Flatley Martin,Edgell David R.,Gloor Gregory B.,Karas Bogumil J.,Patel Rushali R.,Slattery Samuel S..Telomere-to-telomere genome assembly of Phaeodactylum tricornutum[EB/OL].(2025-03-28)[2025-05-21].https://www.biorxiv.org/content/10.1101/2021.05.04.442596.点此复制
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