Nanopore adaptive sampling of a metagenomic sample derived from a human monkeypox case
Nanopore adaptive sampling of a metagenomic sample derived from a human monkeypox case
Abstract In 2022, a series of human monkeypox cases in multiple countries led to the largest and most widespread outbreak outside the known endemic areas. Genomic surveillance is of utmost importance to control such outbreaks. To this end, we performed Nanopore Whole Genome Sequencing of a local monkeypox sample on a PromethION 24. Adaptive sampling was applied for in silico depletion of the human host genome, allowing for the enrichment of low abundance viral DNA without a priori knowledge of sample composition. Nanopore sequencing allowed for high viral genome coverage, tracking of sample composition during sequencing, strain determination, and preliminary assessment of mutational pattern. Nanopore sequencing is a highly versatile method to characterize a virus in real-time without pre-sequencing target enrichment.
Hewel C、Linke M、Kohnen W、Hankeln T、Bikar S-E、Schweiger-Seemann S、Schmidt H、Plachter B、Michel A、Runkel S、Gerber S
Institute of Human Genetics, University Medical Center of the Johannes Gutenberg University MainzInstitute of Human Genetics, University Medical Center of the Johannes Gutenberg University Mainz||SARS-CoV-2 Sequencing Consortium Mainz, University Medical Center of the Johannes Gutenberg University MainzSARS-CoV-2 Sequencing Consortium Mainz, University Medical Center of the Johannes Gutenberg University Mainz||Department of Hygiene and Infection Prevention, University Medical Center of the Johannes Gutenberg University MainzSARS-CoV-2 Sequencing Consortium Mainz, University Medical Center of the Johannes Gutenberg University Mainz||Faculty of Biology, Institute of Organismic and Molecular Evolution, Molecular Genetics & Genome Analysis, Johannes Gutenberg University MainzSARS-CoV-2 Sequencing Consortium Mainz, University Medical Center of the Johannes Gutenberg University Mainz||StarSEQ GmbHInstitute of Human Genetics, University Medical Center of the Johannes Gutenberg University Mainz||SARS-CoV-2 Sequencing Consortium Mainz, University Medical Center of the Johannes Gutenberg University MainzSARS-CoV-2 Sequencing Consortium Mainz, University Medical Center of the Johannes Gutenberg University Mainz||Institute for Virology and Research Center for Immunotherapy (FZI) University Medical Center of the Johannes Gutenberg University MainzSARS-CoV-2 Sequencing Consortium Mainz, University Medical Center of the Johannes Gutenberg University Mainz||Institute for Virology and Research Center for Immunotherapy (FZI) University Medical Center of the Johannes Gutenberg University MainzSARS-CoV-2 Sequencing Consortium Mainz, University Medical Center of the Johannes Gutenberg University Mainz||Medical Management Department, University Medical Center of the Johannes Gutenberg University MainzSARS-CoV-2 Sequencing Consortium Mainz, University Medical Center of the Johannes Gutenberg University Mainz||Transfusion Unit & Test Center, University Medical Center of the Johannes Gutenberg University MainzInstitute of Human Genetics, University Medical Center of the Johannes Gutenberg University Mainz||SARS-CoV-2 Sequencing Consortium Mainz, University Medical Center of the Johannes Gutenberg University Mainz
医学研究方法生物科学研究方法、生物科学研究技术微生物学
Hewel C,Linke M,Kohnen W,Hankeln T,Bikar S-E,Schweiger-Seemann S,Schmidt H,Plachter B,Michel A,Runkel S,Gerber S.Nanopore adaptive sampling of a metagenomic sample derived from a human monkeypox case[EB/OL].(2025-03-28)[2025-05-09].https://www.biorxiv.org/content/10.1101/2023.03.21.533647.点此复制
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