Large-scale implementation of pooled RNA-extraction and RT-PCR for SARS-CoV-2 detection
Large-scale implementation of pooled RNA-extraction and RT-PCR for SARS-CoV-2 detection
Abstract Testing for active SARS-CoV-2 infection is a fundamental tool in the public health measures taken to control the COVID-19 pandemic. Due to the overwhelming use of SARS-CoV-2 RT-PCR tests worldwide, availability of test kits has become a major bottleneck. Here we demonstrate pooling strategies to perform RNA extraction and RT-PCR in pools, significantly increasing throughput while maintaining clinical sensitivity. We implemented the method in a routine clinical diagnosis setting of asymptomatic populations, and already tested 5,464 individuals for SARS-CoV-2 using 731 RNA extraction and RT-PCR kits. We identified six SARS-CoV-2 positive patients corresponding to 0.11% of the tested population.
Oiknine-Djian Esther、Gertler Asaf、Dor Yuval、Salton Maayan、Drier Yotam、Yassour Moran、The Hebrew University-Hadassah COVID-19 diagnosis team、Meshorer Eran、Benedek Gil、Sido Tal、Ben-Ami Roni、Klochendler Agnes、Wolf Dana G.、Rotstein Zeev、Seidel Matan、Gurel-Gurevich Ori、Fogel Irit、Lavi Bruno
Hadassah ¨C Hebrew University Medical CenterHadassah ¨C Hebrew University Medical CenterDepartment of Developmental Biology and Cancer Research, IMRIC, Faculty of Medicine, The Hebrew University of JerusalemDepartment of Biochemistry and Molecular Biology, IMRIC, Faculty of Medicine, The Hebrew University of JerusalemThe Lautenberg Center for Immunology and Cancer Research, IMRIC, Faculty of Medicine, The Hebrew University of JerusalemDepartment of Microbiology and Molecular Genetics, IMRIC, Faculty of Medicine, The Hebrew University of Jerusalem||School of Computer Science and Engineering, The Hebrew University of JerusalemDepartment of Genetics and Edmond and Lily Center for Brain Sciences (ELSC), The Hebrew University of JerusalemHadassah ¨C Hebrew University Medical CenterDepartment of Mathematics, Bar-Ilan UniversityDepartment of Developmental Biology and Cancer Research, IMRIC, Faculty of Medicine, The Hebrew University of JerusalemDepartment of Developmental Biology and Cancer Research, IMRIC, Faculty of Medicine, The Hebrew University of JerusalemHadassah ¨C Hebrew University Medical Center||The Lautenberg Center for Immunology and Cancer Research, IMRIC, Faculty of Medicine, The Hebrew University of JerusalemHadassah ¨C Hebrew University Medical CenterSchool of Mathematical Sciences, Tel Aviv UniversityEinstein Institute of Mathematics, The Hebrew University of JerusalemHadassah ¨C Hebrew University Medical CenterHadassah ¨C Hebrew University Medical Center
医学研究方法预防医学生物科学研究方法、生物科学研究技术
Oiknine-Djian Esther,Gertler Asaf,Dor Yuval,Salton Maayan,Drier Yotam,Yassour Moran,The Hebrew University-Hadassah COVID-19 diagnosis team,Meshorer Eran,Benedek Gil,Sido Tal,Ben-Ami Roni,Klochendler Agnes,Wolf Dana G.,Rotstein Zeev,Seidel Matan,Gurel-Gurevich Ori,Fogel Irit,Lavi Bruno.Large-scale implementation of pooled RNA-extraction and RT-PCR for SARS-CoV-2 detection[EB/OL].(2025-03-28)[2025-05-18].https://www.medrxiv.org/content/10.1101/2020.04.17.20069062.点此复制
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