“Global Transcriptional Response to CRISPR/CAS9-AAV6 Based Genome Editing” Matches Transcriptional Response to Specific Small Molecule Perturbations
“Global Transcriptional Response to CRISPR/CAS9-AAV6 Based Genome Editing” Matches Transcriptional Response to Specific Small Molecule Perturbations
Cromer et al. [1] recently reported global transcriptional changes occuring in cells in response to CRISPR/Cas9 gene editing. Using a CD34+ hematopoietic and progenitor stem cell model, they observed differentially expressed genes enriched for immune, stress and apoptotic processes following treatment with a CRISPR/Cas9-AAV6 genome-editing system. Following treatment with Cas9’s mRNA they observed transcriptional changes enriched for viral response as well as a downregulation of metabolic and cell cycle processes. Similarly, they observed a downregulation of metabolic processes in response to electroporation. Surprisingly, no enrichment for viral response genes was observed following treatment with AAV6 while minor transcriptional changes enriched for DNA damage signature occurred in response to Cas9/sgRNA ribonucleoprotein.
Nordor Akp¨|li V.、Aryee Martin J.、Siwo Geoffrey H.
Institut Curie, PSL Research University, Translational Research Department||Massachusetts General Hospital and Harvard Medical School, Department of Pathology and Center for Cancer Research||Computational Epigenomics Group, Broad Institute of MIT and HarvardMassachusetts General Hospital and Harvard Medical School, Department of Pathology and Center for Cancer Research||Computational Epigenomics Group, Broad Institute of MIT and HarvardCenter for Research Computing, Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame
基础医学生物科学研究方法、生物科学研究技术分子生物学
Nordor Akp¨|li V.,Aryee Martin J.,Siwo Geoffrey H..“Global Transcriptional Response to CRISPR/CAS9-AAV6 Based Genome Editing” Matches Transcriptional Response to Specific Small Molecule Perturbations[EB/OL].(2025-03-28)[2025-05-02].https://www.biorxiv.org/content/10.1101/399311.点此复制
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