A novel α/β T-cell subpopulation defined by recognition of EPCR
A novel α/β T-cell subpopulation defined by recognition of EPCR
Abstract T-cell self-recognition of antigen presenting molecules is led by antigen-dependent or independent mechanisms. The endothelial protein C receptor (EPCR) shares remarkable similarity with CD1d, including a lipid binding cavity. We have identified EPCR-specific α/β T-cells in the peripheral blood of healthy donors. The average frequency in the CD3+ leukocyte pool is comparable to other autoreactive T-cell subsets that specifically bind MHC-like receptors. Alteration of the EPCR lipid cargo, revealed by X-ray diffraction studies, points to a prevalent, yet not exclusive, lipid-independent self-recognition. In addition, we solve the EPCR lipidome, and detect species not yet described as EPCR ligands. These studies report, for the first time, novel recognition by circulating α/β T-cells and provide grounds for EPCR and lipid mediated T-cell restriction.
Saiz Jorge、Mor¨￠n-Garrido Mar¨aa、Dichiara-Rodr¨aguez Gilda、L¨?pez-Sagaseta Jacinto、Barbas Coral、Ram¨arez Natalia、Erausquin Elena
Centro de Metabol¨?mica y Bioan¨￠lisis (CEMBIO), Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanizaci¨?n Montepr¨ancipe, 28660, Boadilla del MonteCentro de Metabol¨?mica y Bioan¨￠lisis (CEMBIO), Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanizaci¨?n Montepr¨ancipe, 28660, Boadilla del MonteUnit of Protein Crystallography and Structural Immunology||Navarra Hospital Complex (CHN)Unit of Protein Crystallography and Structural Immunology||Public University of Navarra (UPNA)||Navarra Hospital Complex (CHN)Centro de Metabol¨?mica y Bioan¨￠lisis (CEMBIO), Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanizaci¨?n Montepr¨ancipe, 28660, Boadilla del MontePublic University of Navarra (UPNA)||Navarra Hospital Complex (CHN)||Unit of Oncohematology, Navarrabiomed, Pamplona||Instituto de Investigaci¨?n Sanitaria de Navarra (IdiSNA)Unit of Protein Crystallography and Structural Immunology||Public University of Navarra (UPNA)||Navarra Hospital Complex (CHN)
基础医学生物科学研究方法、生物科学研究技术分子生物学
Saiz Jorge,Mor¨￠n-Garrido Mar¨aa,Dichiara-Rodr¨aguez Gilda,L¨?pez-Sagaseta Jacinto,Barbas Coral,Ram¨arez Natalia,Erausquin Elena.A novel α/β T-cell subpopulation defined by recognition of EPCR[EB/OL].(2025-03-28)[2025-05-13].https://www.biorxiv.org/content/10.1101/2021.07.01.450412.点此复制
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