有氧运动对肥胖小鼠棕色脂肪mRNA表达谱的影响

Objective Gene Expression Profile was used to screen differentially expressed genes and pathways in Brown Adipose Tissue (BAT) of Obese Mice before and after aerobic exercise, exploring the movement mechanism of activating BAT. Methods 30 C57BL/6J male mice were divided into the control group (N, n=10), high-fat diet control group (OB, n≥6) and high-fat diet exercise group (E, n≥6). Group E was intervened by treadmill training (10m/min，1h/d，6d/w). The food intake and body weight of all the mice were recorded weekly. After four-week experiment, blood lipid and blood glucose were detected; RNA was extracted from BAT of scapula, cDNA was synthesized, and microarray hybridization were scanned to generate gene lists with corresponding fluorescence intensity data. Genes were significantly (P≤0.05) up- or down- regulated more than 2 fold were chosen to do Gene Ontology and enrichment analysis by KOBAS2.0, in order to screen out differentially expressed genes participating in key biological process (BP) and pathway to confirme using RT-PCR. Results Comparing with group N, there are 445 genes were significantly up regulated and 796 were down regulated. The BP of these genes are mainly about the synthesis and metabolism process of lipids, sterol, cholesterol and steroid, as well as the chemotaxis of hemameba and granulocyte. Comparing with group OB, there are 486 genes were significantly up regulated and 286 were down regulated. The BP of these genes are mainly about the synthesis and metabolism process of lipids, sterol, cholesterol and steroid, as well as the metabolism process of carboxylic acid, acetone acid and coenzyme, and antioxidant process. The pathways that aerobic exercise regulate the function of BAT were mainly involving PPAR, AMPK, Insulin and VEGF signaling pathway. Conclusion A series of genes and pathways to regulate glucolipid metabolic and thermogenesis process in BAT could be adjusted by aerobic exercise, in order to improve the activity of BAT, promoting obesity mice to lose weight.were significantly lower than OB; (3) In WAT, comparing with group B, the levels of PGC-1α in group E has a tendency to rise, while group E has a tendery to decline, but there was no significant difference between both of them. In WAT, each intervened group were higher than group OB. Conclusion The levels of UCP-1 and PGC-1α mRNA in WAT and the levels of PGC-1α in BAT by intervention of hypoxia and exercise, and improve the levels of BAT, while improving the activity of BAT in obese mice.