SARS-CoV-2 in wastewater settled solids is associated with COVID-19 cases in a large urban sewershed
SARS-CoV-2 in wastewater settled solids is associated with COVID-19 cases in a large urban sewershed
Abstract Wastewater-based epidemiology (WBE) may be useful for informing public health response to viral diseases like COVID-19 caused by SARS-CoV-2. We quantified SARS-CoV-2 RNA in wastewater influent and primary settled solids in two wastewater treatment plants to inform the pre-analytical and analytical approaches, and to assess whether influent or solids harbored more viral targets. The primary settled solids samples resulted in higher SARS-CoV-2 detection frequencies than the corresponding influent samples. Likewise, SARS-CoV-2 RNA was more readily detected in solids using one-step digital droplet (dd)RT-PCR than with two-step RT-QPCR and two-step ddRT-PCR, likely owing to reduced inhibition with the one-step ddRT-PCR assay. We subsequently analyzed a longitudinal time series of 89 settled solids samples from a single plant for SARS-CoV-2 RNA as well as coronavirus recovery (bovine coronavirus) and fecal strength (pepper mild mottle virus, PMMoV) controls. SARS-CoV-2 RNA targets N1 and N2 concentrations correlate positively and significantly with COVID-19 clinical confirmed case counts in the sewershed. Together, the results demonstrate that measuring SARS-CoV-2 RNA concentrations in settled solids may be a more sensitive approach than measuring SARs-CoV-2 in influent. medrxiv;2020.09.14.20194472v1/UFIG1F1ufig1
Catoe David、Kim Sooyeol、Graham Katherine E.、Loeb Stephanie K.、Wolfe Marlene K.、Wigginton Krista R.、Mendoza Lorelay M.、Boehm Alexandria B.、Sassoubre Lauren M.、Yamahara Kevan M.、Roldan-Hernandez Laura、Li Linlin、Sinnott-Armstrong Nasa
Joint Initiative for Metrology in Biology, SLAC National Accelerator LaboratoryDepartment of Civil & Environmental Engineering, Stanford UniversityDepartment of Civil & Environmental Engineering, Stanford UniversityDepartment of Civil & Environmental Engineering, Stanford UniversityDepartment of Civil & Environmental Engineering, Stanford UniversityDepartment of Civil & Environmental Engineering, University of MichiganDepartment of Civil & Environmental Engineering, Stanford UniversityDepartment of Civil & Environmental Engineering, Stanford UniversityDepartment of Engineering, University of San FranciscoMonterey Bay Aquarium Research InstituteDepartment of Civil & Environmental Engineering, Stanford UniversityCounty of Santa Clara Public Health DepartmentDepartment of Genetics, Stanford University School of Medicine, Stanford, CA Emmett Interdisciplinary Program in Environment and Resources, Stanford University
医学研究方法预防医学环境科学理论环境污染、环境污染防治废物处理、废物综合利用
Catoe David,Kim Sooyeol,Graham Katherine E.,Loeb Stephanie K.,Wolfe Marlene K.,Wigginton Krista R.,Mendoza Lorelay M.,Boehm Alexandria B.,Sassoubre Lauren M.,Yamahara Kevan M.,Roldan-Hernandez Laura,Li Linlin,Sinnott-Armstrong Nasa.SARS-CoV-2 in wastewater settled solids is associated with COVID-19 cases in a large urban sewershed[EB/OL].(2025-03-28)[2025-04-24].https://www.medrxiv.org/content/10.1101/2020.09.14.20194472.点此复制
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