Systematic analysis of C. elegans embryo behavior reveals a stereotyped developmental progression and a rhythmic pattern of behavioral quiescence
Systematic analysis of C. elegans embryo behavior reveals a stereotyped developmental progression and a rhythmic pattern of behavioral quiescence
Abstract Systematic analysis of rich behavioral recordings is being used to uncover how circuits encode complex behaviors. Here we apply the approach to embryos. What are the first embryonic behaviors and how do they evolve as early neurodevelopment ensues? To address these questions, we present a systematic description of behavioral maturation for Caenorhabditis elegans embryos. Posture libraries were derived from a genetically encoded motion capture suit imaged with light-sheet microscopy and annotated using custom semi-automated tracking software (Multiple Hypothesis Hypergraph Tracking; MHHT). Analysis of cell trajectories, postures, and behavioral motifs revealed a stereotyped developmental progression. Early movement is dominated by flipping between dorsal and ventral coiling, which gradually slows into a period of reduced motility. Late-stage embryos exhibit sinusoidal waves of dorsoventral bends, prolonged bouts of directed motion, and a rhythmic pattern of pausing, which we designate slow wave twitch (SWT). Synaptic transmission is required for late-stage motion but not for early flipping or the intervening inactive phase. A high-throughput behavioral assay and calcium imaging revealed that SWT is elicited by the rhythmic activity of a quiescence-promoting neuron (RIS). Similar periodic quiescent states are seen prenatally in divergent animals and may play an important role in promoting normal developmental outcomes.
Ardiel Evan L.、Harvey Brandon J.、Nurrish Stephen、Lauziere Andrew、Kaplan Joshua M.、Shroff Hari、Xu Stephen、Christensen Ryan
Department of Molecular Biology, Massachusetts General Hospital||Department of Neurobiology, Harvard Medical SchoolNational Institute of Biomedical Imaging and Bioengineering, National Institutes of Health||Department of Neurology, Emory UniversityDepartment of Molecular Biology, Massachusetts General Hospital||Department of Neurobiology, Harvard Medical SchoolNational Institute of Biomedical Imaging and Bioengineering, National Institutes of Health||Department of Mathematics, University of MarylandDepartment of Molecular Biology, Massachusetts General Hospital||Department of Neurobiology, Harvard Medical SchoolNational Institute of Biomedical Imaging and Bioengineering, National Institutes of Health||Fellows Program, Marine Biological LaboratoryNational Institute of Biomedical Imaging and Bioengineering, National Institutes of HealthNational Institute of Biomedical Imaging and Bioengineering, National Institutes of Health
细胞生物学遗传学分子生物学
Ardiel Evan L.,Harvey Brandon J.,Nurrish Stephen,Lauziere Andrew,Kaplan Joshua M.,Shroff Hari,Xu Stephen,Christensen Ryan.Systematic analysis of C. elegans embryo behavior reveals a stereotyped developmental progression and a rhythmic pattern of behavioral quiescence[EB/OL].(2025-03-28)[2025-05-21].https://www.biorxiv.org/content/10.1101/2021.12.09.471955.点此复制
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