Locally-invasive, castrate-resistant prostate cancer in a Pten/Trp53 double knockout mouse model of prostate cancer monitored with non-invasive bioluminescent imaging
Locally-invasive, castrate-resistant prostate cancer in a Pten/Trp53 double knockout mouse model of prostate cancer monitored with non-invasive bioluminescent imaging
Abstract Here we have improved an existing mouse model of prostate cancer based on prostate-specific deletion of Pten and Trp53 by incorporating a Cre-activatable luciferase reporter. By coupling the deletion of those genes to the activation of a luciferase reporter, we were able to monitor tumor burden non-invasively over time. We show that, consistent with previous reports, deletion of both Pten and Trp53 on a C57BL/6 background accelerates tumor growth and results in both the loss of androgen receptor expression and castrate resistant tumors as compared with loss of Pten alone. Loss of Trp53 results in the development of sarcomatoid histology and the expression of markers of epithelial-to-mesenchymal transition Zeb1 and vimentin, with kinetics and penetrance dependent on whether one or both alleles of Trp53 were deleted. Homozygous deletion of Trp53 and Pten resulted in uniformly lethal disease by 25 weeks. While we were able to detect locally invasive disease in the peritoneal cavity in aggressive tumors from the double knockout mice, we were unable to detect lymphatic or hematogenous metastatic disease in lymph nodes or at distant sites.
Svensson Robert、Cohen Michael B.、Yong Courtney、Henry Michael D.、Vanneste Marion、Brown James A.、Breheny Patrick、Moose Devon L.、Bannick Nadine
Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of IowaDepartment of Pathology, Wake Forest School of MedicineDepartment of Urology, Carver College of Medicine, University of IowaDepartment of Urology, Carver College of Medicine, University of Iowa||Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa||Department of Pathology, Carver College of Medicine, University of Iowa||Department of Radiation Oncology, Carver College of Medicine, University of Iowa||Holden Comprehensive Cancer CenterDepartment of Molecular Physiology and Biophysics, Carver College of Medicine, University of IowaDepartment of Urology, Carver College of Medicine, University of IowaDepartment of Biostatistics, College of Public Health, University of IowaDepartment of Molecular Physiology and Biophysics, Carver College of Medicine, University of IowaDepartment of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa
医学研究方法基础医学肿瘤学
Svensson Robert,Cohen Michael B.,Yong Courtney,Henry Michael D.,Vanneste Marion,Brown James A.,Breheny Patrick,Moose Devon L.,Bannick Nadine.Locally-invasive, castrate-resistant prostate cancer in a Pten/Trp53 double knockout mouse model of prostate cancer monitored with non-invasive bioluminescent imaging[EB/OL].(2025-03-28)[2025-05-11].https://www.biorxiv.org/content/10.1101/2020.04.23.057661.点此复制
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