No substantial pre-existing B cell immunity against SARS-CoV-2 in healthy adults
No substantial pre-existing B cell immunity against SARS-CoV-2 in healthy adults
Summary Pre-existing immunity against SARS-CoV-2 may have critical implications for our understanding of COVID-19 susceptibility and severity. Various studies recently provided evidence of pre-existing T cell immunity against SARS-CoV-2 in unexposed individuals. In contrast, the presence and clinical relevance of a pre-existing B cell immunity remains to be fully elucidated. Here, we provide a detailed analysis of the B cell response to SARS-CoV-2 in unexposed individuals. To this end, we extensively investigated the memory B cell response to SARS-CoV-2 in 150 adults sampled pre-pandemically. Comprehensive screening of donor plasma and purified IgG samples for binding and neutralization in various functional assays revealed no substantial activity against SARS-CoV-2 but broad reactivity to endemic betacoronaviruses. Moreover, we analyzed antibody sequences of 8,174 putatively SARS-CoV-2-reactive B cells on a single cell level and generated and tested 158 monoclonal antibodies. None of the isolated antibodies displayed relevant binding or neutralizing activity against SARS-CoV-2. Taken together, our results show no evidence of relevant pre-existing antibody and B cell immunity against SARS-CoV-2 in unexposed adults.
Koch Manuel、Korenkov Michael、Kl¨1ver Michael、Di Cristanziano Veronica、Vanshylla Kanika、Kreer Christoph、Klein Florian、D?hling Sabrina、Gruell Henning、Gathof Birgit、Schlotz Maike、Worczinski Johanna、Halwe Sandro、Ercanoglu Meryem Seda、Janicki Hanna、Zehner Matthias、Becker Stephan、Poopalasingam Nareshkumar、Gieselmann Lutz、Detmer Susanne
Center for Molecular Medicine Cologne (CMMC), University of Cologne||Institute for Dental Research and Oral Musculoskeletal Biology and Center for Biochemistry, University of CologneLaboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of CologneInstitute of Virology, Philipps University Marburg||German Center for Infection ResearchLaboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of CologneLaboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of CologneLaboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of CologneLaboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne||German Center for Infection Research||Center for Molecular Medicine Cologne (CMMC), University of CologneLaboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of CologneLaboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne||German Center for Infection ResearchInstitute of Transfusion Medicine, Faculty of Medicine and University Hospital CologneLaboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of CologneLaboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of CologneInstitute of Virology, Philipps University Marburg||German Center for Infection ResearchLaboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of CologneLaboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of CologneLaboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne||German Center for Infection ResearchInstitute of Virology, Philipps University Marburg||German Center for Infection ResearchLaboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of CologneLaboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne||German Center for Infection ResearchLaboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne
医药卫生理论医学研究方法基础医学
Koch Manuel,Korenkov Michael,Kl¨1ver Michael,Di Cristanziano Veronica,Vanshylla Kanika,Kreer Christoph,Klein Florian,D?hling Sabrina,Gruell Henning,Gathof Birgit,Schlotz Maike,Worczinski Johanna,Halwe Sandro,Ercanoglu Meryem Seda,Janicki Hanna,Zehner Matthias,Becker Stephan,Poopalasingam Nareshkumar,Gieselmann Lutz,Detmer Susanne.No substantial pre-existing B cell immunity against SARS-CoV-2 in healthy adults[EB/OL].(2025-03-28)[2025-05-05].https://www.biorxiv.org/content/10.1101/2021.09.08.459398.点此复制
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