Bacterial contact induces polar plug disintegration to mediate whipworm egg hatching
Bacterial contact induces polar plug disintegration to mediate whipworm egg hatching
Abstract The bacterial microbiota promotes the life cycle of the intestine-dwelling whipworm Trichuris by mediating hatching of parasite eggs ingested by the mammalian host. Despite the enormous disease burden associated with Trichuris colonization, the mechanisms underlying this transkingdom interaction have been obscure. Here, we used a multiscale microscopy approach to define the structural events associated with bacteria-mediated hatching of eggs for the murine model parasite Trichuris muris. Through the combination of scanning electron microscopy (SEM) and serial block face SEM (SBFSEM), we visualized the outer surface morphology of the shell and generated 3D structures of the egg and larva during the hatching process. These images revealed that exposure to hatching-inducing bacteria catalyzed asymmetric degradation of the polar plugs prior to exit by the larva. Although unrelated bacteria induced similar loss of electron density and dissolution of the structural integrity of the plugs, egg hatching was most efficient in the presence of bacteria that bound poles with high density such as Staphylococcus aureus. Consistent with the ability of taxonomically distant bacteria to induce hatching, additional results suggest chitinase released from larva within the eggs degrade the plugs from the inside instead of enzymes produced by bacteria in the external environment. These findings define at ultrastructure resolution the evolutionary adaptation of a parasite for the microbe-rich environment of the mammalian gut.
Liang Feng-Xia、Robertson Amicha、Sall Joseph、Venzon Mericien、Torres Victor J.、Olivas Janet J.、Thur Rafaela Saes、Zheng Xuhui、Cammer Michael、Cadwell Ken、Nejsum Peter、Antao Noelle、Bethony Jeffrey
Microscopy Laboratory, Division of Advanced Research Technologies, New York University Langone Health||Department of Cell Biology, NYU Grossman School of MedicineDepartment of Microbiology, New York University Grossman School of MedicineMicroscopy Laboratory, Division of Advanced Research Technologies, New York University Langone Health||Department of Cell Biology, NYU Grossman School of MedicineDepartment of Microbiology, New York University Grossman School of MedicineDepartment of Microbiology, New York University Grossman School of Medicine||Antimicrobial-Resistant Pathogens Program, NYU Langone HealthDepartment of Microbiology, New York University Grossman School of MedicineDepartment of Microbiology, Immunology and Tropical Medicine, George Washington UniversityDepartment of Microbiology, New York University Grossman School of MedicineMicroscopy Laboratory, Division of Advanced Research Technologies, New York University Langone Health||Department of Cell Biology, NYU Grossman School of MedicineDivision of Gastroenterology and Hepatology, Department of Medicine, University of Pennsylvania Perelman School of Medicine||Department of Systems Pharmacology and Translational Therapeutics, University of Pennsylvania Perelman School of Medicine||Department of Pathology and Laboratory Medicine, University of Pennsylvania Perelman School of MedicineDepartment of Clinical Medicine, Aarhus UniversityDepartment of Cell Biology, NYU Grossman School of MedicineDepartment of Microbiology, Immunology and Tropical Medicine, George Washington University
微生物学细胞生物学分子生物学
Liang Feng-Xia,Robertson Amicha,Sall Joseph,Venzon Mericien,Torres Victor J.,Olivas Janet J.,Thur Rafaela Saes,Zheng Xuhui,Cammer Michael,Cadwell Ken,Nejsum Peter,Antao Noelle,Bethony Jeffrey.Bacterial contact induces polar plug disintegration to mediate whipworm egg hatching[EB/OL].(2025-03-28)[2025-05-24].https://www.biorxiv.org/content/10.1101/2023.03.13.532458.点此复制
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