New isolates refine the ecophysiology of the Roseobacter CHAB-I-5 lineage
New isolates refine the ecophysiology of the Roseobacter CHAB-I-5 lineage
Abstract The CHAB-I-5 cluster is a pelagic lineage that can comprise a significant proportion of all roseobacters in surface oceans and have predicted roles in biogeochemical cycling via heterotrophy, aerobic anoxygenic photosynthesis (AAnP), CO oxidation, DMSP degradation, and other metabolisms. Though cultures of CHAB-I-5 have been reported, none have been explored and the best known representative, strain SB2, was lost from culture after obtaining the genome sequence. We have isolated two new CHAB-I-5 representatives, strains US3C007 and FZCC0083, and assembled complete, circularized genomes with 98.7% and 92.5% average nucleotide identities with the SB2 genome. Comparison of these three with 49 other unique CHAB-I-5 metagenome-assembled and single-cell genomes indicated that the cluster represents a genus with two species, and we identified subtle differences in genomic content between the two species subclusters. Metagenomic recruitment from over fourteen hundred samples expanded their known global distribution and highlighted both isolated strains as representative members of the clade. FZCC0083 grew over twice as fast as US3C007 and over a wider range of temperatures. The axenic culture of US3C007 occurs as pleomorphic cells with most exhibiting a coccobacillus/vibrioid shape. We propose the name Thalassovivens spotae, gen nov., sp. nov. for the type strain US3C007T.
Lanclos V. Celeste、Cheng Chuankai、Hider Cole J.、Coelho Jordan T.、Kojima Conner Y.、Thrash J. Cameron、Yang Mingyu、Xie Mei、Zhao Yanlin、Cleveland Catie S.、Barnes Shelby J.、Feng Xiaoyuan、Luo Haiwei
Department of Biological Sciences, University of Southern CaliforniaDepartment of Biological Sciences, University of Southern CaliforniaDepartment of Biological Sciences, University of Southern CaliforniaDepartment of Biological Sciences, University of Southern CaliforniaDepartment of Biological Sciences, University of Southern CaliforniaDepartment of Biological Sciences, University of Southern CaliforniaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry UniversitySimon F. S. Li Marine Science Laboratory, School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong||Shenzhen Research Institute, The Chinese University of Hong KongFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry UniversityDepartment of Biological Sciences, University of Southern CaliforniaDepartment of Biological Sciences, University of Southern CaliforniaSimon F. S. Li Marine Science Laboratory, School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong||Shenzhen Research Institute, The Chinese University of Hong KongSimon F. S. Li Marine Science Laboratory, School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong||Shenzhen Research Institute, The Chinese University of Hong Kong
微生物学分子生物学环境生物学
Lanclos V. Celeste,Cheng Chuankai,Hider Cole J.,Coelho Jordan T.,Kojima Conner Y.,Thrash J. Cameron,Yang Mingyu,Xie Mei,Zhao Yanlin,Cleveland Catie S.,Barnes Shelby J.,Feng Xiaoyuan,Luo Haiwei.New isolates refine the ecophysiology of the Roseobacter CHAB-I-5 lineage[EB/OL].(2025-03-28)[2025-04-30].https://www.biorxiv.org/content/10.1101/2024.05.28.596239.点此复制
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