Mapping enzyme catalysis with metabolomic biosensing
Mapping enzyme catalysis with metabolomic biosensing
Abstract Enzymes are represented across a vast space of protein sequences and structural forms and have activities that far exceed the best chemical catalysts; however, engineering them to have novel or enhanced activity is limited by technologies for sensing product formation. Here, we describe a general and scalable approach for characterizing enzyme activity that uses the metabolism of the host cell as a biosensor by which to infer product formation. Since different products consume different molecules in their synthesis, they perturb host metabolism in unique ways that can be measured by mass spectrometry. This provides a general way by which to sense product formation, to discover unexpected products and map the effects of mutagenesis.
Kennedy Robert T.、Abate Adam R.、Payne Emory M.、Modavi Cyrus、Palmer Claire M.、Tao Nannan、Cornett Dale S.、Liu Leqian、Alper Hal S.、Chang Kai-Chun、Xu Linfeng
Department of Chemistry, University of MichiganDepartment of Bioengineering and Therapeutic sciences, University of California||Chan Zuckerberg BiohubDepartment of Chemistry, University of MichiganDepartment of Bioengineering and Therapeutic sciences, University of CaliforniaInstitute for Cellular and Molecular Biology, The University of Texas at AustinBruker Nano SurfacesBruker DaltonicsDepartment of Bioengineering and Therapeutic sciences, University of CaliforniaInstitute for Cellular and Molecular Biology, The University of Texas at Austin||McKetta Department of Chemical Engineering, The University of Texas at AustinDepartment of Bioengineering and Therapeutic sciences, University of CaliforniaDepartment of Bioengineering and Therapeutic sciences, University of California
生物科学研究方法、生物科学研究技术生物化学生物物理学
Kennedy Robert T.,Abate Adam R.,Payne Emory M.,Modavi Cyrus,Palmer Claire M.,Tao Nannan,Cornett Dale S.,Liu Leqian,Alper Hal S.,Chang Kai-Chun,Xu Linfeng.Mapping enzyme catalysis with metabolomic biosensing[EB/OL].(2025-03-28)[2025-04-26].https://www.biorxiv.org/content/10.1101/2021.10.15.464597.点此复制
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