Structure of the Core of the Type Three Secretion System Export Apparatus
Structure of the Core of the Type Three Secretion System Export Apparatus
Summary Export of proteins through type three secretion systems is critical for bacterial motility and virulence of many major bacterial pathogens. Three putative integral membrane proteins (FliP/FliQ/FliR) are suggested to form the core of an export gate in the inner membrane, but their structure, assembly and location within the final nanomachine remain unclear. We here present the structure of this complex at 4.2 ? by cryo-electron microscopy. None of the subunits adopt canonical integral membrane protein topologies and common helix-turn-helix structural elements allow them to form a helical assembly with 5:4:1 stoichiometry. Fitting of the structure into reconstructions of intact secretion systems localize the export gate as a core component of the periplasmic portion of the machinery, and cross-linking experiments confirm this observation. This study thereby identifies the export gate as a key element of the secretion channel and implies that it primes the helical architecture of the components assembling downstream. One Sentence SummaryThe core of the T3SS export gate forms a supra-membrane helical assembly
Johnson Steven、Mebrhatu Mehari Tesfazgi、Gault Joseph、Dietsche Tobias、Robinson Carol V.、Kuhlen Lucas、Ganief Tariq、Wagner Samuel、Macek Boris、Caesar Joseph、Abrusci Patrizia、Deme Justin、Lea Susan M.
Sir William Dunn School of Pathology, University of Oxford, South Parks RoadSection of Cellular and Molecular Microbiology, Interfaculty Institute of Microbiology and Infection Medicine (IMIT), University of T¨1bingenDepartment of Chemistry, University of OxfordSection of Cellular and Molecular Microbiology, Interfaculty Institute of Microbiology and Infection Medicine (IMIT), University of T¨1bingenDepartment of Chemistry, University of OxfordSir William Dunn School of Pathology, University of Oxford, South Parks Road||Department of Chemistry, University of OxfordProteome Center T¨1bingen, University of T¨1bingenSection of Cellular and Molecular Microbiology, Interfaculty Institute of Microbiology and Infection Medicine (IMIT), University of T¨1bingen||German Center for Infection Research, Partner-site T¨1bingen, Elfriede-Aulhorn-StrProteome Center T¨1bingen, University of T¨1bingenSir William Dunn School of Pathology, University of Oxford, South Parks Road||Central Oxford Structural Microscopy and Imaging Centre, University of OxfordSir William Dunn School of Pathology, University of Oxford, South Parks RoadSir William Dunn School of Pathology, University of Oxford, South Parks Road||Central Oxford Structural Microscopy and Imaging Centre, University of OxfordSir William Dunn School of Pathology, University of Oxford, South Parks Road||Central Oxford Structural Microscopy and Imaging Centre, University of Oxford
分子生物学微生物学
Johnson Steven,Mebrhatu Mehari Tesfazgi,Gault Joseph,Dietsche Tobias,Robinson Carol V.,Kuhlen Lucas,Ganief Tariq,Wagner Samuel,Macek Boris,Caesar Joseph,Abrusci Patrizia,Deme Justin,Lea Susan M..Structure of the Core of the Type Three Secretion System Export Apparatus[EB/OL].(2025-03-28)[2025-05-12].https://www.biorxiv.org/content/10.1101/249128.点此复制
评论