Cryo-EM structures of human RNA polymerase I
Cryo-EM structures of human RNA polymerase I
Abstract RNA polymerase I (Pol I) specifically synthesizes ribosomal RNA. Pol I upregulation is linked to cancer, while mutations in the Pol I machinery lead to developmental disorders. Here, we report the cryo-EM structure of elongating human Pol I at 2.7 ? resolution. In the exit tunnel, we observe a double-stranded RNA helix that may support Pol I processivity. Our structure confirms that human Pol I consists of 13 subunits with only one subunit forming the Pol I stalk. Additionally, the structure of human Pol I in complex with the initiation factor RRN3 at 3.1 ? resolution reveals stalk flipping upon RRN3 binding. We also observe an inactivated state of human Pol I bound to an open DNA scaffold at 3.3 ? resolution. Lastly, the high-resolution structure of human Pol I allows mapping of disease-related mutations that can aid understanding of disease etiology.
Murciano Brice、Girbig Mathias、Gr?tsch Helga、Lafita Aleix、M¨1ller Christoph W.、Baudin Florence、Misiaszek Agata D.
European Molecular Biology Laboratory (EMBL), Structural and Computational Biology UnitEuropean Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit||Candidate for joint PhD degree from EMBL and Heidelberg University, Faculty of BiosciencesEuropean Molecular Biology Laboratory (EMBL), Structural and Computational Biology UnitEuropean Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome CampusEuropean Molecular Biology Laboratory (EMBL), Structural and Computational Biology UnitEuropean Molecular Biology Laboratory (EMBL), Structural and Computational Biology UnitEuropean Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit||Candidate for joint PhD degree from EMBL and Heidelberg University, Faculty of Biosciences
基础医学分子生物学生物科学研究方法、生物科学研究技术
Murciano Brice,Girbig Mathias,Gr?tsch Helga,Lafita Aleix,M¨1ller Christoph W.,Baudin Florence,Misiaszek Agata D..Cryo-EM structures of human RNA polymerase I[EB/OL].(2025-03-28)[2025-08-03].https://www.biorxiv.org/content/10.1101/2021.05.31.446457.点此复制
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