A Connectome of the Adult Drosophila Central Brain
A Connectome of the Adult Drosophila Central Brain
Abstract The neural circuits responsible for behavior remain largely unknown. Previous efforts have reconstructed the complete circuits of small animals, with hundreds of neurons, and selected circuits for larger animals. Here we (the FlyEM project at Janelia and collaborators at Google) summarize new methods and present the complete circuitry of a large fraction of the brain of a much more complex animal, the fruit fly Drosophila melanogaster. Improved methods include new procedures to prepare, image, align, segment, find synapses, and proofread such large data sets; new methods that define cell types based on connectivity in addition to morphology; and new methods to simplify access to a large and evolving data set. From the resulting data we derive a better definition of computational compartments and their connections; an exhaustive atlas of cell examples and types, many of them novel; detailed circuits for most of the central brain; and exploration of the statistics and structure of different brain compartments, and the brain as a whole. We make the data public, with a web site and resources specifically designed to make it easy to explore, for all levels of expertise from the expert to the merely curious. The public availability of these data, and the simplified means to access it, dramatically reduces the effort needed to answer typical circuit questions, such as the identity of upstream and downstream neural partners, the circuitry of brain regions, and to link the neurons defined by our analysis with genetic reagents that can be used to study their functions. Note: In the next few weeks, we will release a series of papers with more involved discussions. One paper will detail the hemibrain reconstruction with more extensive analysis and interpretation made possible by this dense connectome. Another paper will explore the central complex, a brain region involved in navigation, motor control, and sleep. A final paper will present insights from the mushroom body, a center of multimodal associative learning in the fly brain.
Scott Anne K.、Hayworth Kenneth J.、Rubin Gerald M.、Katz William、Tenshaw Emily、Ito Kei、Sobeski Margaret A.、Saalfeld Stephan、Meinertzhagen Ian、Hess Harald F.、Cook Michael、Finley Samantha、Knecht Christopher J. Jr.、Umayam Lowell、Hubbard Philip、Kawase Takashi、Lindsey Larry、Otsuna Hideo、Troutman Eric T.、Xu C. Shan、Lu Zhiyuan、Takemura Shin-ya、Huang Gary、Shinomiya Kazunori、Hopkins Gary Patrick、Kovalyak Julie、Lohff Alanna、Maldonado Charli、Manley Emily A.、Mooney Caroline、Okeoma Nneoma、Phillips Elliott E.、Rampally Neha、Ribeiro Caitlin、Scott Ashley L.、Tokhi Temour、Yang Tansy、Horne Jane Anne、Padilla Nicholas、Smith Natalie L.、Suleiman Alia、Swift Jackie、Alvarado Chelsea X.、Tarnogorska Dorota、Cheatham Natasha、Neace Erika R.、Borycz Jolanta A、Rivlin Patricia K.、Schlegel Philipp、Lauchie Shirley A.、Leavitt Laramie、Ito Masayoshi、Dreher Marisa、Berg Stuart、McLin Sari、Smith Kelsey、Kirk Nicole A.、Januszewski Michal、Clements Jody、Kainmueller Dagmar、Zhao Ting、Svirskas Robert、Francis Audrey、Bailey Dennis A.、Canino Brandon S.、Dolafi Tom、Ackerman David、Kim SungJin、Rymer Jon Thomson、Olbris Donald J.、Li Feng、Takemura Satoko、Eubanks Bryon、Sammons Megan、Plaza Stephen M.、Ballinger Samantha、Neubarth Nicole、Fairbanks Kelli、Jain Viren、Patrick Christopher、Maitin-Shepard Jeremy、Goldammer Jens、Duclos Octave、Wolff Tanya、Forknall Nora、Talebi Iris、Ndama Miatta、Scheffer Louis K.、Blakely Tim、Khairy Khaled A.、Li Peter H.、Ogundeyi Omotara、Jayaraman Vivek、George Reed、Joyce Emily M.、Bogovic John、Robertson Madelaine K、Ordish Christopher、Phillips Emily M.、Walsh John J.、Parekh Ruchi、Shinomiya Aya、Ryan Sean M.、Smith Claire、Paterson Tyler
Janelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMI||University of Tokyo, Institute for Quantitative Biosciences||Institute of Zoology, Biocenter Cologne, University of CologneJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMI||Life Sciences Center, Dalhousie UniversityJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIGoogle ResearchJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMI||Life Sciences Center, Dalhousie UniversityJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMI||Life Sciences Center, Dalhousie UniversityJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMILife Sciences Center, Dalhousie UniversityJanelia Research Campus, HHMIJanelia Research Campus, HHMILife Sciences Center, Dalhousie UniversityJanelia Research Campus, HHMIDepartment of Zoology, University of CambridgeJanelia Research Campus, HHMIGoogle ResearchJanelia Research Campus, HHMI||University of Tokyo, Institute for Quantitative BiosciencesJanelia Research Campus, HHMIJanelia Research Campus, HHMILife Sciences Center, Dalhousie UniversityJanelia Research Campus, HHMIJanelia Research Campus, HHMIGoogle ResearchJanelia Research Campus, HHMIJanelia Research Campus, HHMI||Max Delbrueck Center for Molecular MedicineJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMITwo Six LabsJanelia Research Campus, HHMIGoogle ResearchJanelia Research Campus, HHMIGoogle ResearchJanelia Research Campus, HHMI||Institute of Zoology, Biocenter Cologne, University of CologneJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIGoogle ResearchJanelia Research Campus, HHMI||Department of Developmental Neurobiology, St. Jude Childrens Research HospitalGoogle ResearchJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMILife Sciences Center, Dalhousie UniversityJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMIJanelia Research Campus, HHMI
生物科学研究方法、生物科学研究技术昆虫学生物科学现状、生物科学发展
Scott Anne K.,Hayworth Kenneth J.,Rubin Gerald M.,Katz William,Tenshaw Emily,Ito Kei,Sobeski Margaret A.,Saalfeld Stephan,Meinertzhagen Ian,Hess Harald F.,Cook Michael,Finley Samantha,Knecht Christopher J. Jr.,Umayam Lowell,Hubbard Philip,Kawase Takashi,Lindsey Larry,Otsuna Hideo,Troutman Eric T.,Xu C. Shan,Lu Zhiyuan,Takemura Shin-ya,Huang Gary,Shinomiya Kazunori,Hopkins Gary Patrick,Kovalyak Julie,Lohff Alanna,Maldonado Charli,Manley Emily A.,Mooney Caroline,Okeoma Nneoma,Phillips Elliott E.,Rampally Neha,Ribeiro Caitlin,Scott Ashley L.,Tokhi Temour,Yang Tansy,Horne Jane Anne,Padilla Nicholas,Smith Natalie L.,Suleiman Alia,Swift Jackie,Alvarado Chelsea X.,Tarnogorska Dorota,Cheatham Natasha,Neace Erika R.,Borycz Jolanta A,Rivlin Patricia K.,Schlegel Philipp,Lauchie Shirley A.,Leavitt Laramie,Ito Masayoshi,Dreher Marisa,Berg Stuart,McLin Sari,Smith Kelsey,Kirk Nicole A.,Januszewski Michal,Clements Jody,Kainmueller Dagmar,Zhao Ting,Svirskas Robert,Francis Audrey,Bailey Dennis A.,Canino Brandon S.,Dolafi Tom,Ackerman David,Kim SungJin,Rymer Jon Thomson,Olbris Donald J.,Li Feng,Takemura Satoko,Eubanks Bryon,Sammons Megan,Plaza Stephen M.,Ballinger Samantha,Neubarth Nicole,Fairbanks Kelli,Jain Viren,Patrick Christopher,Maitin-Shepard Jeremy,Goldammer Jens,Duclos Octave,Wolff Tanya,Forknall Nora,Talebi Iris,Ndama Miatta,Scheffer Louis K.,Blakely Tim,Khairy Khaled A.,Li Peter H.,Ogundeyi Omotara,Jayaraman Vivek,George Reed,Joyce Emily M.,Bogovic John,Robertson Madelaine K,Ordish Christopher,Phillips Emily M.,Walsh John J.,Parekh Ruchi,Shinomiya Aya,Ryan Sean M.,Smith Claire,Paterson Tyler.A Connectome of the Adult Drosophila Central Brain[EB/OL].(2025-03-28)[2025-07-01].https://www.biorxiv.org/content/10.1101/2020.01.21.911859.点此复制
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