Knockout of zebrafish desmin genes does not cause skeletal muscle degeneration but alters calcium flux
Knockout of zebrafish desmin genes does not cause skeletal muscle degeneration but alters calcium flux
Abstract Desmin is a muscle-specific intermediate filament protein that has fundamental role in muscle structure and force transmission. Whereas human desmin protein is encoded by a single gene, two desmin paralogs (desma and desmb) exist in zebrafish. Desma and desmb show differential spatiotemporal expression during zebrafish embryonic and larval development, being similarly expressed in skeletal muscle until hatching, after which expression of desmb shifts to gut smooth muscle. We generated knockout (KO) mutant lines carrying loss-of-function mutations for each gene by using CRISPR/Cas9. Mutants are viable and fertile, and lack obvious skeletal muscle, heart or intestinal defects. In contrast to morphants, knockout of each gene did not cause any overt muscular phenotype, but did alter calcium flux in myofibres. These results point to a possible compensation mechanism in these mutant lines generated by targeting nonsense mutations to the first coding exon.
Kurekci Gulsum Kayman、Unsal Seyda、Hughes Simon M.、Gizer Merve、Dincer Pervin R.、Ergin Bora、Mangit Ecem Kural、D¨1z Niloufar Boustanabadimaralan、Saglam Berk、Koyunlar Cansu、Korkusuz Petek、Purali Nuhan、Talim Beril、Uyanik Ismail
Department of Medical Biology, Hacettepe University Faculty of MedicineDepartment of Medical Biology, Hacettepe University Faculty of Medicine||Department of Cancer and Inflammation, The Institute of Molecular Medicine, University of Southern DenmarkRandall Centre for Cell and Molecular Biophysics, New Hunt?ˉs House, Guy?ˉs Campus, King?ˉs College LondonDepartment of Stem Cell Sciences, Hacettepe University Graduate School of Health SciencesDepartment of Medical Biology, Hacettepe University Faculty of MedicineDepartment of Biophysics, Hacettepe University Faculty of MedicineDepartment of Medical Biology, Hacettepe University Faculty of Medicine||Laboratory Animals Research and Application Centre, Hacettepe UniversityDepartment of Medical Biology, Hacettepe University Faculty of MedicineDepartment of Biophysics, Hacettepe University Faculty of MedicineDepartment of Medical Biology, Hacettepe University Faculty of Medicine||Department of HematologyHistology and Embryology, Hacettepe University Faculty of MedicineDepartment of Cancer and Inflammation, The Institute of Molecular Medicine, University of Southern DenmarkDepartment of Pediatrics, Pathology Unit, Hacettepe University Faculty of MedicineDepartment of Electrical and Electronics Engineering, Hacettepe University Faculty of Engineering
遗传学生理学分子生物学
Kurekci Gulsum Kayman,Unsal Seyda,Hughes Simon M.,Gizer Merve,Dincer Pervin R.,Ergin Bora,Mangit Ecem Kural,D¨1z Niloufar Boustanabadimaralan,Saglam Berk,Koyunlar Cansu,Korkusuz Petek,Purali Nuhan,Talim Beril,Uyanik Ismail.Knockout of zebrafish desmin genes does not cause skeletal muscle degeneration but alters calcium flux[EB/OL].(2025-03-28)[2025-04-27].https://www.biorxiv.org/content/10.1101/2020.10.16.342485.点此复制
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