Neuronal wiring diagram of an adult brain
Neuronal wiring diagram of an adult brain
Abstract Connections between neurons can be mapped by acquiring and analyzing electron microscopic (EM) brain images. In recent years, this approach has been applied to chunks of brains to reconstruct local connectivity maps that are highly informative, yet inadequate for understanding brain function more globally. Here, we present the first neuronal wiring diagram of a whole adult brain, containing 5×107 chemical synapses between ～130,000 neurons reconstructed from a female Drosophila melanogaster. The resource also incorporates annotations of cell classes and types, nerves, hemilineages, and predictions of neurotransmitter identities. Data products are available by download, programmatic access, and interactive browsing and made interoperable with other fly data resources. We show how to derive a projectome, a map of projections between regions, from the connectome. We demonstrate the tracing of synaptic pathways and the analysis of information flow from inputs (sensory and ascending neurons) to outputs (motor, endocrine, and descending neurons), across both hemispheres, and between the central brain and the optic lobes. Tracing from a subset of photoreceptors all the way to descending motor pathways illustrates how structure can uncover putative circuit mechanisms underlying sensorimotor behaviors. The technologies and open ecosystem of the FlyWire Consortium set the stage for future large-scale connectome projects in other species.
Ogedengbe Oluwaseun、Castro Manuel、Kruk Krzysztof、Yang Runzhe、Sorek Marissa、Lu Ran、Mu Shang、Wu Jingpeng、Kemnitz Nico、Eckstein Nils、Funke Jan、Bock Davi D.、Matsliah Arie、Schlegel Philipp、Yu Szi-chieh、Lin Albert、Costa Marta、Kuehner Kai、Ih Dodam、Macrina Thomas、Dorkenwald Sven、Popovych Sergiy、Gager Jay、Silversmith Will、Yin Yijie、Collman Forrest、Lee Kisuk、Jia Zhen、Morey Ryan、McKellar Claire E.、Brittain Derrick、Eichler Katharina、Nehoran Barak、Bland Doug、the FlyWire Consortium、Deutsch David、Jordan Chris S.、Murthy Mala、Bae J. Alexander、Schneider-Mizell Casey、Perlman Eric、Seung H. Sebastian、Halageri Akhilesh、Sterling Amy R、Bates Alexander Shakeel、Jefferis Gregory S.X.E.、Mitchell Eric
Princeton Neuroscience Institute, Princeton UniversityPrinceton Neuroscience Institute, Princeton UniversityEyewirePrinceton Neuroscience Institute, Princeton University||Computer Science Department, Princeton UniversityPrinceton Neuroscience Institute, Princeton University||EyewirePrinceton Neuroscience Institute, Princeton UniversityPrinceton Neuroscience Institute, Princeton UniversityPrinceton Neuroscience Institute, Princeton UniversityPrinceton Neuroscience Institute, Princeton UniversityJanelia Research Campus, Howard Hughes Medical InstituteJanelia Research Campus, Howard Hughes Medical InstituteDepartment of Neurological Sciences, University of VermontPrinceton Neuroscience Institute, Princeton UniversityNeurobiology Division, MRC Laboratory of Molecular Biology||Drosophila Connectomics Group, Department of Zoology, University of CambridgePrinceton Neuroscience Institute, Princeton UniversityPrinceton Neuroscience Institute, Princeton University||Center for the Physics of Biological Function, Princeton UniversityDrosophila Connectomics Group, Department of Zoology, University of CambridgePrinceton Neuroscience Institute, Princeton UniversityPrinceton Neuroscience Institute, Princeton UniversityPrinceton Neuroscience Institute, Princeton University||Computer Science Department, Princeton UniversityPrinceton Neuroscience Institute, Princeton University||Computer Science Department, Princeton UniversityPrinceton Neuroscience Institute, Princeton University||Computer Science Department, Princeton UniversityPrinceton Neuroscience Institute, Princeton UniversityPrinceton Neuroscience Institute, Princeton UniversityDrosophila Connectomics Group, Department of Zoology, University of CambridgeAllen Institute for Brain SciencePrinceton Neuroscience Institute, Princeton University||Brain & Cognitive Sciences Department, Massachusetts Institute of TechnologyPrinceton Neuroscience Institute, Princeton UniversityPrinceton Neuroscience Institute, Princeton UniversityPrinceton Neuroscience Institute, Princeton UniversityAllen Institute for Brain ScienceDrosophila Connectomics Group, Department of Zoology, University of CambridgePrinceton Neuroscience Institute, Princeton University||Computer Science Department, Princeton UniversityPrinceton Neuroscience Institute, Princeton UniversityPrinceton Neuroscience Institute, Princeton University||Department of Neurobiology, University of HaifaPrinceton Neuroscience Institute, Princeton UniversityPrinceton Neuroscience Institute, Princeton UniversityPrinceton Neuroscience Institute, Princeton University||Electrical and Computer Engineering Department, Princeton UniversityAllen Institute for Brain ScienceYikes LLCPrinceton Neuroscience Institute, Princeton University||Computer Science Department, Princeton UniversityPrinceton Neuroscience Institute, Princeton UniversityPrinceton Neuroscience Institute, Princeton University||EyewireNeurobiology Division, MRC Laboratory of Molecular Biology||Drosophila Connectomics Group, Department of Zoology, University of Cambridge||Harvard Medical School||Centre for Neural Circuits and Behaviour, The University of OxfordNeurobiology Division, MRC Laboratory of Molecular Biology||Drosophila Connectomics Group, Department of Zoology, University of CambridgePrinceton Neuroscience Institute, Princeton University
生物科学研究方法、生物科学研究技术昆虫学计算技术、计算机技术
Ogedengbe Oluwaseun,Castro Manuel,Kruk Krzysztof,Yang Runzhe,Sorek Marissa,Lu Ran,Mu Shang,Wu Jingpeng,Kemnitz Nico,Eckstein Nils,Funke Jan,Bock Davi D.,Matsliah Arie,Schlegel Philipp,Yu Szi-chieh,Lin Albert,Costa Marta,Kuehner Kai,Ih Dodam,Macrina Thomas,Dorkenwald Sven,Popovych Sergiy,Gager Jay,Silversmith Will,Yin Yijie,Collman Forrest,Lee Kisuk,Jia Zhen,Morey Ryan,McKellar Claire E.,Brittain Derrick,Eichler Katharina,Nehoran Barak,Bland Doug,the FlyWire Consortium,Deutsch David,Jordan Chris S.,Murthy Mala,Bae J. Alexander,Schneider-Mizell Casey,Perlman Eric,Seung H. Sebastian,Halageri Akhilesh,Sterling Amy R,Bates Alexander Shakeel,Jefferis Gregory S.X.E.,Mitchell Eric.Neuronal wiring diagram of an adult brain[EB/OL].(2025-03-28)[2025-05-11].https://www.biorxiv.org/content/10.1101/2023.06.27.546656.点此复制
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