A novel role of 3’,5’-cAMP in the regulation of actin cytoskeleton in Arabidopsis
A novel role of 3’,5’-cAMP in the regulation of actin cytoskeleton in Arabidopsis
Abstract The role of cyclic adenosine monophosphate (3’,5’-cAMP) in plants is not well understood, and here, we report a novel role of 3’,5’-cAMP in regulating the actin cytoskeleton. The 3’,5’-cAMP treatment affects the thermal stability of 51 proteins, including a vegetative actin isoform, ACTIN2. Consistent with the above results, the increase in 3’,5’-cAMP levels, obtained either by feeding or by chemical modulation of 3’,5’-cAMP metabolism, is sufficient to partially rescue the short hypocotyl phenotype of the actin2 actin7 mutant, severely compromised in actin level. No such complementation was measured for a positional isomer of 3’,5’-cAMP, 2’,3’-cAMP, attesting to the specificity of 3’,5’-cAMP treatment. Moreover, supplementation of 3’,5’-cAMP partly counters the activity of an actin-depolymerizing drug latrunculin B. In vitro characterization of the 3’,5’-cAMP – actin interaction argues against the direct binding. Instead, based on the proteomics characterization of the act2act7 mutant supplemented with 3’,5’-cAMP, we hypothesize that 3’,5’-cAMP affects cytoskeleton dynamic by modulation of calcium signaling, and actin binding proteins.
Chodasiewicz Monika、Franz Peter、Luzarowski Marcin、Tsiavaliaris Georgios、Skirycz Aleksandra、Saluja Manny、Childs Dorothee、Figueroa Nicol¨￠s E.、Moreno Juan C.、Masiuk Aleksandra、Sampathkumar Arun
Max-Planck-Institute of Molecular Plant Physiology||Center for Desert Agriculture, Biological and Environmental Science & Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST)Institute for Biophysical Chemistry, Hannover Medical SchoolMax-Planck-Institute of Molecular Plant PhysiologyInstitute for Biophysical Chemistry, Hannover Medical SchoolMax-Planck-Institute of Molecular Plant Physiology||Boyce Thompson Institute (BTI)Boyce Thompson Institute (BTI)European Molecular Biology Laboratory (EMBL) HeidelbergCenter for Desert Agriculture, Biological and Environmental Science & Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST)Max-Planck-Institute of Molecular Plant Physiology||Center for Desert Agriculture, Biological and Environmental Science & Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST)Max-Planck-Institute of Molecular Plant PhysiologyMax-Planck-Institute of Molecular Plant Physiology
植物学生物化学分子生物学
Chodasiewicz Monika,Franz Peter,Luzarowski Marcin,Tsiavaliaris Georgios,Skirycz Aleksandra,Saluja Manny,Childs Dorothee,Figueroa Nicol¨￠s E.,Moreno Juan C.,Masiuk Aleksandra,Sampathkumar Arun.A novel role of 3’,5’-cAMP in the regulation of actin cytoskeleton in Arabidopsis[EB/OL].(2025-03-28)[2025-04-30].https://www.biorxiv.org/content/10.1101/2022.01.31.478439.点此复制
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