Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth
Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth
Summary The capacity to fully replace teeth continuously makes zebrafish an attractive model to explore regeneration and tooth development. The requirement of attachment bone for the appearance of replacement teeth has been hypothesized but not yet investigated. The transcription factor sp7 (osterix) is known in mammals to play an important role during odontoblast differentiation and root formation. Here we study tooth replacement in the absence of attachment bone using sp7 zebrafish mutants. We analysed the pattern of tooth replacement at different stages of development and demonstrated that in zebrafish lacking sp7, attachment bone is never present, independent of the stage of tooth development or fish age, yet replacement is not interrupted. Without bone of attachment we observed abnormal orientation of teeth, and abnormal connection of pulp cavities of predecessor and replacement teeth. Mutants lacking sp7 show arrested dentinogenesis, with non-polarization of odontoblasts and only a thin layer of dentin deposited. Osteoclast activity was observed in sp7 mutants; due to the lack of bone of attachment, remodelling was diminished but nevertheless present along the pharyngeal bone. We conclude that tooth replacement is ongoing in the sp7 mutant despite poor differentiation and defective attachment. Without bone of attachment tooth orientation and pulp organization are compromised.
Huysseune A、Kague E、Lubiana T、Hammond C、Campos CL、Passos-Bueno MR、Soenens M、Witten P.E、Robson Brown K、Fisher S
Evolutionary Developmental Biology, Ghent UniversityDepartment of Physiology, Pharmacology and Neuroscience, University of Bristol||Centro de Pesquisa sobre o Genoma Humano e C¨|lulas Tronco, Departamento de Gen¨|tica e Biologia Evolutiva, Instituto de Bioci¨oncias, Universidade de S?o PauloCentro de Pesquisa sobre o Genoma Humano e C¨|lulas Tronco, Departamento de Gen¨|tica e Biologia Evolutiva, Instituto de Bioci¨oncias, Universidade de S?o PauloDepartment of Physiology, Pharmacology and Neuroscience, University of BristolCentro de Pesquisa sobre o Genoma Humano e C¨|lulas Tronco, Departamento de Gen¨|tica e Biologia Evolutiva, Instituto de Bioci¨oncias, Universidade de S?o PauloCentro de Pesquisa sobre o Genoma Humano e C¨|lulas Tronco, Departamento de Gen¨|tica e Biologia Evolutiva, Instituto de Bioci¨oncias, Universidade de S?o PauloEvolutionary Developmental Biology, Ghent UniversityEvolutionary Developmental Biology, Ghent UniversitySchool of Archaeology and Anthropology, University of BristolDepartment of Pharmacology and Experimental Therapeutics, Boston University School of Medicine
动物学遗传学分子生物学
Huysseune A,Kague E,Lubiana T,Hammond C,Campos CL,Passos-Bueno MR,Soenens M,Witten P.E,Robson Brown K,Fisher S.Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth[EB/OL].(2025-03-28)[2025-05-06].https://www.biorxiv.org/content/10.1101/259085.点此复制
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