TATTOO-seq delineates spatial and cell type-specific regulatory programs during limb patterning
TATTOO-seq delineates spatial and cell type-specific regulatory programs during limb patterning
Abstract The coordinated differentiation of progenitor cells into specialized cell types and their spatial organization into distinct domains is central to embryogenesis. Here, we applied a new unbiased spatially resolved single-cell transcriptomics method to identify the genetic programs that underlie the emergence of specialized cell types during limb development and their integration in space. We uncovered combinations of transcription factors whose expression patterns are predominantly associated with cell type specification or spatial position, enabling the deconvolution of cell fate and position identity. We demonstrate that the embryonic limb undergoes a complex multi-scale re-organization upon perturbation of one of its spatial organizing centers, including the loss of specific cell populations, specific alterations in the molecular identities of other pre-existing cell states and changes in their relative spatial distribution. Altogether, our study shows how multi-dimensional single-cell and spatially resolved molecular atlases could reveal the interconnected genetic networks that regulate the intricacies of organogenesis and its reorganization upon genetic alterations.
Bastide S¨|bastien、Schmutz Sandrine、Novault Sophie、Chomsky Elad、Loe-Mie Yann、Saudemont Baptiste、Marlow Heather、Spitz Fran?ois、Tanay Amos
(Epi)genomics of Animal Development, Department of Developmental and Stem Cell Biology, Institut Pasteur||¨|cole Doctorale ?°Complexit¨| du Vivant?±, Sorbonne Universit¨|||Department of Human Genetics, The University of ChicagoCytometry and Biomarkers, Center for Technological Resources and Research, Institut Pasteur.Cytometry and Biomarkers, Center for Technological Resources and Research, Institut Pasteur.Department of Computer Science and Applied Mathematics, Weizmann Institute||Department of Biological Regulation, Weizmann Institute(Epi)genomics of Animal Development, Department of Developmental and Stem Cell Biology, Institut Pasteur||Hub de Bioinformatique et Biostatistique, D¨|partement Biologie Computationnelle, Institut Pasteur(Epi)genomics of Animal Development, Department of Developmental and Stem Cell Biology, Institut Pasteur(Epi)genomics of Animal Development, Department of Developmental and Stem Cell Biology, Institut Pasteur||Department of Organismal Biology and Anatomy, The University of Chicago(Epi)genomics of Animal Development, Department of Developmental and Stem Cell Biology, Institut Pasteur||Department of Human Genetics, The University of ChicagoDepartment of Computer Science and Applied Mathematics, Weizmann Institute
生物科学研究方法、生物科学研究技术分子生物学细胞生物学
Bastide S¨|bastien,Schmutz Sandrine,Novault Sophie,Chomsky Elad,Loe-Mie Yann,Saudemont Baptiste,Marlow Heather,Spitz Fran?ois,Tanay Amos.TATTOO-seq delineates spatial and cell type-specific regulatory programs during limb patterning[EB/OL].(2025-03-28)[2025-05-26].https://www.biorxiv.org/content/10.1101/2022.03.20.482385.点此复制
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