Cilia regulate meiotic recombination in zebrafish
Cilia regulate meiotic recombination in zebrafish
Abstract Meiosis is essential for evolution and genetic diversity in almost all sexual eukaryotic organisms. The mechanisms of meiotic recombination, such as synapsis, have been extensively investigated. However, it is still unclear whether signals from the cytoplasm or even outside of the cell can regulate the meiosis process. Cilia are microtubule-based structures that protrude from cell surface and function as signaling hubs to sense extracellular signals. Here, we reported an unexpected and critical role of cilia during meiotic recombination. During gametogenesis of zebrafish, cilia were specifically present in the prophase stages of both spermatocytes and primary oocytes. By developing a novel germ cell-specific CRISPR/Cas9 system, we demonstrated that germ cell-specific depletion of ciliary genes resulted in compromised double strand break repair, reduced crossover formation, and increased germ cell apoptosis. Our study reveals a previously undiscovered role for cilia during meiosis and suggests that extracellular signals may regulate meiotic recombination via this particular organelle.
Chen Zhe、Xie Haibo、Wang Xiaosi、Li Lanqin、Zhu Junwen、Jin Minjun、Zhao Chengtian、Sun Yonghua、Kang Yunsi
Institute of Evolution & Marine Biodiversity, Ocean University of ChinaInstitute of Evolution & Marine Biodiversity, Ocean University of China||Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology||Sars-Fang Centre, Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Innovation Academy for Seed Design, Hubei Hongshan Laboratory, Chinese Academy of SciencesInstitute of Evolution & Marine Biodiversity, Ocean University of China||Sars-Fang Centre, Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Innovation Academy for Seed Design, Hubei Hongshan Laboratory, Chinese Academy of Sciences||College of Advanced Agricultural Sciences, University of Chinese Academy of SciencesInstitute of Evolution & Marine Biodiversity, Ocean University of China||Sars-Fang Centre, Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of ChinaInstitute of Evolution & Marine Biodiversity, Ocean University of China||Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology||Sars-Fang Centre, Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Innovation Academy for Seed Design, Hubei Hongshan Laboratory, Chinese Academy of Sciences||College of Advanced Agricultural Sciences, University of Chinese Academy of SciencesInstitute of Evolution & Marine Biodiversity, Ocean University of China||Sars-Fang Centre, Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China
遗传学细胞生物学分子生物学
Chen Zhe,Xie Haibo,Wang Xiaosi,Li Lanqin,Zhu Junwen,Jin Minjun,Zhao Chengtian,Sun Yonghua,Kang Yunsi.Cilia regulate meiotic recombination in zebrafish[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2022.03.05.482955.点此复制
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