Metabolomics and lipidomics in C. elegans using a single sample preparation
Metabolomics and lipidomics in C. elegans using a single sample preparation
Abstract Comprehensive metabolomic and lipidomic mass spectrometry methods are in increasing demand, for instance in research related to nutrition and aging. The nematode C. elegans is a key model organism in these fields, due to the large repository of available C. elegans mutants and their convenient natural lifespan. Here, we describe a robust and sensitive analytical method for the semi-quantitative analysis of >100 polar (metabolomics) and >1000 apolar (lipidomics) metabolites in C. elegans, using a single sample preparation. Our method is capable of reliably detecting a wide variety of biologically relevant metabolic aberrations in, for instance, glycolysis and the TCA cycle, pyrimidine metabolism and complex lipid biosynthesis. In conclusion, we provide a powerful analytical tool that maximizes metabolic data yield from a single sample. Graphical abstractbiorxiv;2020.07.06.190017v1/UFIG1F1ufig1
Pras-Raves Mia L.、Janssens Georges E.、Schomakers Bauke V.、Vervaart Martin A.T.、Luyf Angela C.、Molenaars Marte、Smith Reuben L.、van Kampen Antoine H. C.、Elfrink Hyung L.、Sterken Mark G.、Gao Arwen W.、Vaz Fr¨|d¨|ric M.、Houtkooper Riekelt H.、Kammenga Jan E.、van Weeghel Michel
Laboratory Genetic Metabolic Diseases, Amsterdam Gastroenterology and Metabolism, Amsterdam Cardiovascular Sciences, Amsterdam UMC, University of Amsterdam||Core Facility Metabolomics, Amsterdam UMC, University of Amsterdam||Bioinformatics Laboratory, Amsterdam Public Health research institute, Amsterdam UMC, University of AmsterdamLaboratory Genetic Metabolic Diseases, Amsterdam Gastroenterology and Metabolism, Amsterdam Cardiovascular Sciences, Amsterdam UMC, University of AmsterdamLaboratory Genetic Metabolic Diseases, Amsterdam Gastroenterology and Metabolism, Amsterdam Cardiovascular Sciences, Amsterdam UMC, University of Amsterdam||Core Facility Metabolomics, Amsterdam UMC, University of AmsterdamLaboratory Genetic Metabolic Diseases, Amsterdam Gastroenterology and Metabolism, Amsterdam Cardiovascular Sciences, Amsterdam UMC, University of Amsterdam||Core Facility Metabolomics, Amsterdam UMC, University of AmsterdamBioinformatics Laboratory, Amsterdam Public Health research institute, Amsterdam UMC, University of AmsterdamLaboratory Genetic Metabolic Diseases, Amsterdam Gastroenterology and Metabolism, Amsterdam Cardiovascular Sciences, Amsterdam UMC, University of AmsterdamLaboratory Genetic Metabolic Diseases, Amsterdam Gastroenterology and Metabolism, Amsterdam Cardiovascular Sciences, Amsterdam UMC, University of AmsterdamBioinformatics Laboratory, Amsterdam Public Health research institute, Amsterdam UMC, University of AmsterdamLaboratory Genetic Metabolic Diseases, Amsterdam Gastroenterology and Metabolism, Amsterdam Cardiovascular Sciences, Amsterdam UMC, University of Amsterdam||Core Facility Metabolomics, Amsterdam UMC, University of AmsterdamLaboratory of Nematology, Wageningen University and ResearchLaboratory Genetic Metabolic Diseases, Amsterdam Gastroenterology and Metabolism, Amsterdam Cardiovascular Sciences, Amsterdam UMC, University of Amsterdam||Laboratory of Integrative Systems Physiology, Institute of Bioengineering, ¨|cole Polytechnique F¨|d¨|rale de LausanneLaboratory Genetic Metabolic Diseases, Amsterdam Gastroenterology and Metabolism, Amsterdam Cardiovascular Sciences, Amsterdam UMC, University of Amsterdam||Core Facility Metabolomics, Amsterdam UMC, University of AmsterdamLaboratory Genetic Metabolic Diseases, Amsterdam Gastroenterology and Metabolism, Amsterdam Cardiovascular Sciences, Amsterdam UMC, University of AmsterdamLaboratory of Nematology, Wageningen University and ResearchLaboratory Genetic Metabolic Diseases, Amsterdam Gastroenterology and Metabolism, Amsterdam Cardiovascular Sciences, Amsterdam UMC, University of Amsterdam||Core Facility Metabolomics, Amsterdam UMC, University of Amsterdam
生物科学研究方法、生物科学研究技术生物化学分子生物学
Pras-Raves Mia L.,Janssens Georges E.,Schomakers Bauke V.,Vervaart Martin A.T.,Luyf Angela C.,Molenaars Marte,Smith Reuben L.,van Kampen Antoine H. C.,Elfrink Hyung L.,Sterken Mark G.,Gao Arwen W.,Vaz Fr¨|d¨|ric M.,Houtkooper Riekelt H.,Kammenga Jan E.,van Weeghel Michel.Metabolomics and lipidomics in C. elegans using a single sample preparation[EB/OL].(2025-03-28)[2025-08-14].https://www.biorxiv.org/content/10.1101/2020.07.06.190017.点此复制
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