Generation of chimeric respiratory viruses with altered tropism by coinfection of influenza A virus and respiratory syncytial virus
Generation of chimeric respiratory viruses with altered tropism by coinfection of influenza A virus and respiratory syncytial virus
Summary Interactions between co-circulating respiratory viruses are recognized for their impact on viral transmission and clinical outcomes. However, the consequences of these virus-virus interactions at the cellular level are unclear. We coinfected human lung cells with influenza A virus (IAV) and respiratory syncytial virus (RSV). Super-resolution microscopy combined with live-cell imaging and scanning electron microscopy identified extracellular and membrane-associated filamentous structures, likely composed of elements of both IAV and RSV virions. Cryo-electron tomography confirmed the presence of chimeric virus particles exhibiting glycoproteins and ribonucleoproteins of both parental viruses. Functional assays revealed chimeric particles facilitate IAV infection in cells depleted of IAV receptors, demonstrating expanded tropism. Our observations define a previously unknown interaction that is likely to affect virus pathogenesis and have profound implications for infection biology.
Vijayakrishnan Swetha、Haney Joanne、Bhella David、Streetley James、Dee Kieran、Clarke Mairi、Mullin Margaret、Goldfarb Daniel Max、Murcia Pablo R、Carter Stephen D
MRC-University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of GlasgowMRC-University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of GlasgowMRC-University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow||Scottish Centre for Macromolecular Imaging, College of Medical, Veterinary and Life Sciences, University of GlasgowScottish Centre for Macromolecular Imaging, College of Medical, Veterinary and Life Sciences, University of GlasgowMRC-University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of GlasgowScottish Centre for Macromolecular Imaging, College of Medical, Veterinary and Life Sciences, University of GlasgowGlasgow Imaging Facility, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of GlasgowMRC-University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of GlasgowMRC-University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of GlasgowMRC-University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow
基础医学微生物学分子生物学
Vijayakrishnan Swetha,Haney Joanne,Bhella David,Streetley James,Dee Kieran,Clarke Mairi,Mullin Margaret,Goldfarb Daniel Max,Murcia Pablo R,Carter Stephen D.Generation of chimeric respiratory viruses with altered tropism by coinfection of influenza A virus and respiratory syncytial virus[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2021.08.16.456460.点此复制
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