Lipid transfer from plants to arbuscular mycorrhiza fungi
Lipid transfer from plants to arbuscular mycorrhiza fungi
Abstract Arbuscular mycorrhiza (AM) symbioses contribute to global carbon cycles as plant hosts divert up to 20% of photosynthate to the obligate biotrophic fungi. Previous studies suggested carbohydrates as the only form of carbon transferred to the fungi. However, de novo fatty acid (FA) synthesis has not been observed in AM fungi in absence of the plant. In a forward genetic approach, we identified two Lotus japonicus mutants defective in AM-specific paralogs of lipid biosynthesis genes (KASI and GPAT6). These mutants perturb fungal development and accumulation of emblematic fungal 16:1ω5 FAs. Using isotopolog profiling we demonstrate that 13C patterns of fungal FAs recapitulate those of wild-type hosts, indicating cross-kingdom lipid transfer from plants to fungi. This transfer of labelled FAs was not observed for the AM-specific lipid biosynthesis mutants. Thus, growth and development of beneficial AM fungi is not only fueled by sugars but depends on lipid transfer from plant hosts.
Wewer Vera、Bucerius Simone L.、Delaux Pierre-Marc、Klingl Verena、D?rmann Peter、Keymer Andreas、Huber Claudia、Parniske Martin、Wang Trevor L.、Gutjahr Caroline、Eisenreich Wolfgang、Pimprikar Priya、Brands Mathias、R?penack-Lahaye Edda von
Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of BonnFaculty of Biology, Genetics, LMU Munich, Biocenter MartinsriedCentre National de la Recherche ScientifiqueFaculty of Biology, Genetics, LMU Munich, Biocenter MartinsriedInstitute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of BonnFaculty of Biology, Genetics, LMU Munich, Biocenter MartinsriedBiochemistry, Technical University Munich (TUM)Faculty of Biology, Genetics, LMU Munich, Biocenter MartinsriedJohn Innes Centre, Norwich Research ParkFaculty of Biology, Genetics, LMU Munich, Biocenter MartinsriedBiochemistry, Technical University Munich (TUM)Faculty of Biology, Genetics, LMU Munich, Biocenter MartinsriedInstitute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of BonnFaculty of Biology, Plant Sciences, LMU Munich, Biocenter Martinsried
生物科学研究方法、生物科学研究技术植物学微生物学
Wewer Vera,Bucerius Simone L.,Delaux Pierre-Marc,Klingl Verena,D?rmann Peter,Keymer Andreas,Huber Claudia,Parniske Martin,Wang Trevor L.,Gutjahr Caroline,Eisenreich Wolfgang,Pimprikar Priya,Brands Mathias,R?penack-Lahaye Edda von.Lipid transfer from plants to arbuscular mycorrhiza fungi[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/143883.点此复制
评论