Improved Nanopore full-length cDNA sequencing by PCR-suppression
Improved Nanopore full-length cDNA sequencing by PCR-suppression
Full-length transcript sequencing remains a main goal of RNA sequencing. However, even the application of long-read sequencing technologies such as Oxford Nanopore Technologies still fail to yield full-length transcript sequencing for a significant portion of sequenced reads. Since these technologies can sequence reads that are far longer than the longest known processed transcripts, the lack of efficiency to obtain full-length transcripts from good quality RNAs stems from library preparation inefficiency rather than the presence of degraded RNA molecules. It has previously been shown that addition of inverted terminal repeats in cDNA during reverse transcription followed by single-primer PCR creates a PCR suppression effect that prevents amplification of short molecules thus enriching the library for longer transcripts. We adapted this method for Nanopore cDNA library preparation and show that not only is PCR efficiency increased but gene body coverage is dramatically improved. The results show that implementation of this simple strategy will result in better quality full-length RNA sequencing data and make full-length transcript sequencing possible for most of sequenced reads.
Oikonomopoulos Spyridon、Wang Yu Chang、Ragoussis Jiannis、Bayega Anthony
生物科学研究方法、生物科学研究技术分子生物学生物工程学
Oikonomopoulos Spyridon,Wang Yu Chang,Ragoussis Jiannis,Bayega Anthony.Improved Nanopore full-length cDNA sequencing by PCR-suppression[EB/OL].(2025-03-28)[2025-05-25].https://www.biorxiv.org/content/10.1101/2022.07.28.501892.点此复制
评论