Glutathione contributes to plant defense against parasitic cyst nematodes
Glutathione contributes to plant defense against parasitic cyst nematodes
Abstract Cyst nematodes (CNs) are an important group of root-infecting sedentary endoparasites that severely damage many crop plants worldwide. An infective CN juvenile enters the host’s roots and migrates towards the vascular cylinder, where it induces the formation of syncytial feeding cells, which nourish the CN throughout its parasitic stages. Here, we examined the role of glutathione (L-γ-glutamyl-L-cysteinylglycine, GSH) in Arabidopsis thaliana upon infection with the CN Heterodera schachtii. Arabidopsis lines with mutations pad2, cad2, or zir1 in the glutamate–cysteine ligase (GSH1) gene, which encodes the first enzyme in the glutathione biosynthetic pathway, displayed enhanced CN susceptibility, but susceptibility was reduced for rax1, another GSH1 allele. Biochemical analysis revealed differentially altered thiol levels in these mutants that was independent of nematode infection. All GSH-deficient mutants exhibited impaired activation of defense marker genes as well as genes for biosynthesis of the antimicrobial compound camalexin early in infection. Further analysis revealed a link between glutathione-mediated plant susceptibility to CN infection and the production of camalexin upon nematode infection. These results suggest that GSH levels affects plant susceptibility to CN by fine-tuning the balance between the cellular redox environment and the production of compounds related to defense against infection.
Damm Anika、Kopriva Stanislav、Grundler Florian M.W.、Siddique Shahid、Koprivova Anna、Chopra Divykriti、M¨1ller-Sch¨1ssele Stefanie、Meyer Andreas J.、Hasan M. Shamim
Institute of Crop Science and Resource Conservation (INRES), Molecular Phytomedicine, University of Bonn, INRESInstitute for Plant Sciences, Cluster of Excellence on Plant Sciences, University of CologneInstitute of Crop Science and Resource Conservation (INRES), Molecular Phytomedicine, University of Bonn, INRESInstitute of Crop Science and Resource Conservation (INRES), Molecular Phytomedicine, University of Bonn, INRES||Department of Entomology and Nematology, University of CaliforniaInstitute for Plant Sciences, Cluster of Excellence on Plant Sciences, University of CologneInstitute of Crop Science and Resource Conservation (INRES), Molecular Phytomedicine, University of Bonn, INRESInstitute of Crop Science and Resource Conservation (INRES), Chemical Signalling, University of BonnInstitute of Crop Science and Resource Conservation (INRES), Chemical Signalling, University of BonnInstitute of Crop Science and Resource Conservation (INRES), Molecular Phytomedicine, University of Bonn, INRES||Department of Plant Pathology, Faculty of Agriculture, Hajee Mohammad Danesh Science and Technology University
生物化学植物学分子生物学
Damm Anika,Kopriva Stanislav,Grundler Florian M.W.,Siddique Shahid,Koprivova Anna,Chopra Divykriti,M¨1ller-Sch¨1ssele Stefanie,Meyer Andreas J.,Hasan M. Shamim.Glutathione contributes to plant defense against parasitic cyst nematodes[EB/OL].(2025-03-28)[2025-05-18].https://www.biorxiv.org/content/10.1101/2021.09.23.461554.点此复制
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