Homologous chromosomes in asexual rotifer Adineta vaga suggest automixis
Homologous chromosomes in asexual rotifer Adineta vaga suggest automixis
ABSTRACT The several hundreds of species of bdelloid rotifers are notorious because they represent an ancient clade comprising only asexual lineages1. Moreover, most bdelloid species have the ability to withstand complete desiccation and high doses of ionizing radiation, being able to repair their DNA after massive genome breakage2. To better understand the impact of long-term asexuality and DNA breakage on genome evolution, a telomere-to-tolemere reference genome assembly of a bdelloid species is critical3, 4. Here we present the first, high quality chromosome-scale genome assembly for the bdelloid A. vaga validated using three complementary assembly procedures combined with chromosome conformation capture (Hi-C) data. The different assemblies reveal the same genome architecture and using fluorescent in situ hybridization (FISH), we demonstrate that the A. vaga genome is composed of six pairs of homologous chromosomes, compatible with meiosis. Moreover, the synteny between homoeologous (or ohnologous) chromosomes is also preserved, confirming their paleotetraploidy. The diploid genome structure of A. vaga and the presence of very long homozygous tracts show that recombination between homologous chromosomes occurs in this ancient asexual scandal, either during DSB repair or during meiotic pairing. These homozygosity tracts are mainly observed towards the chromosome ends in the clonal A. vaga suggesting signatures of a parthenogenetic mode of reproduction equivalent to central fusion automixis, in which homologous chromosomes are not segregated during the meiotic division.
Houtain Antoine、Cariou Marie、Nicolas Emilien、Guiglielmoni Nad¨¨ge、Kozlowski Djampa KL、Virgo Julie、Wincker Patrick、Hallet Bernard、Koszul Romain、Flot Jean-Fran?ois、Danchin Etienne GJ、Derzelle Alessandro、Baudry Lyam、Simion Paul、Gaudray Florence Rodriguez、Terwagne Matthieu、Van Doninck Karine、Noel Benjamin、Narayan Jitendra、Marbouty Martial、Limasset Antoine
Universit¨| de Namur, LEGE, URBECentre International de Recherche en Infectiologie (CIRI), Univ Lyon, Inserm, U1111, Universit¨|e claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, 46 All¨|e d?ˉItalieUniversit¨| de Namur, LEGE, URBEUniversit¨| libre de Bruxelles (ULB), Evolutionary Biology and EcologyINRAE/Universit¨| Co?te d?ˉAzur/CNRS, Institut Sophia AgrobiotechUniversit¨| de Namur, LEGE, URBEG¨|nomique M¨|tabolique, Genoscope, Institut Franc?ois Jacob, CEA, CNRS, Univ Evry, Universit¨| Paris-SaclayUniversit¨| Catholique de Louvain (UCLouvain), Louvain Institute of Biomolecular Science and Technology (LIBST), Croix du Sud 4/5Institut Pasteur, Unit¨| R¨|gulation Spatiale des G¨|nomesUniversit¨| libre de Bruxelles (ULB), Evolutionary Biology and Ecology||Interuniversity Institute of Bioinformatics in Brussels - (IB)2INRAE/Universit¨| Co?te d?ˉAzur/CNRS, Institut Sophia AgrobiotechUniversit¨| de Namur, LEGE, URBEInstitut Pasteur, Unit¨| R¨|gulation Spatiale des G¨|nomesUniversit¨| de Namur, LEGE, URBEUniversit¨| libre de Bruxelles (ULB), Evolutionary Biology and EcologyUniversit¨| de Namur, LEGE, URBEUniversit¨| de Namur, LEGE, URBEG¨|nomique M¨|tabolique, Genoscope, Institut Franc?ois Jacob, CEA, CNRS, Univ Evry, Universit¨| Paris-SaclayUniversit¨| de Namur, LEGE, URBEInstitut Pasteur, Unit¨| R¨|gulation Spatiale des G¨|nomesUniversit¨| libre de Bruxelles (ULB), Evolutionary Biology and Ecology||Universit¨| de Lille, CNRS, UMR 9189 - CRIStAL, 59655 Villeneuve-d?ˉAscq
遗传学细胞生物学动物学
Houtain Antoine,Cariou Marie,Nicolas Emilien,Guiglielmoni Nad¨¨ge,Kozlowski Djampa KL,Virgo Julie,Wincker Patrick,Hallet Bernard,Koszul Romain,Flot Jean-Fran?ois,Danchin Etienne GJ,Derzelle Alessandro,Baudry Lyam,Simion Paul,Gaudray Florence Rodriguez,Terwagne Matthieu,Van Doninck Karine,Noel Benjamin,Narayan Jitendra,Marbouty Martial,Limasset Antoine.Homologous chromosomes in asexual rotifer Adineta vaga suggest automixis[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2020.06.16.155473.点此复制
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