HOXBLINC 敲除小鼠模型的构建

Objective: In this study, a mouse model of HOXBLINC knockout was successfully constructed using CRISPR/Cas9 gene editing technology, aiming to provide a reliable animal platform for exploring the function of long non-coding RNA HOXBLINC in hematology and leukemia. Experimental methods: A CRISPR/Cas9 system targeting HOXBLINC was designed and constructed, and gene-edited mice were obtained by microinjection. PCR genotype identification and qPCR expression analysis were used to verify the knockout efficiency of HOXBLINC. Multi-generational breeding and genetic analysis were used to confirm the stable genetic characteristics of knockout mouse germlines. Results: The HOXBLINC knockout mouse model was successfully constructed and had stable genetic characteristics, which laid an important foundation for the subsequent in-depth study of the physiological function of HOXBLINC in animals and its mechanism of action in hematologic diseases.