低温控制的水相双水体系用于金银花提取后绿原酸和芦丁的分配

he aqueous two-phase system (ATPS) shows the advantages of high extraction efficiency and good purity in extraction of active components from Chinese herbs. However, conventional ATPS often contains high concentrations of salts, and when the herb is located in the salt-rich phase, dissolution of organic components in this phas is deterred, resulting in poor mass transfer efficiency and long extraction period. By using an acetonitrile-water mixture of single phase, chlorogenic acid (CGA) and Rutin were extracted from honeysuckle, followed by enrichment of the CGA and rutin in the bottom phase of the ATPS mainly consisting of acetonitrile and water at low temperature (3 C). The extraction factors such as temperature, extraction time, material-to-liquid ratio, and acetonitrile fraction were optimized, and the kinetic and thermodynamic properties of the extraction process were analyzed. Both the extraction efficiency for CGA and rutin were above 90%. Adding a small amount of ethyl acetate (EA) and triethylamine helped phase separation and improved their distribution coefficients to 22.8 and 22.6, respectively. This low-temperature-controlled ATPS provides a new way for extraction and enrichment of natural products.