独脚金内酯通路与蓝光受体互作调控拟南芥分枝

Branching, developed from axillary buds, is a critical agronomic morphological trait in the aerial parts of plants, exerting significant impacts on plant architecture and crop yield. The regulation of plant branching is a complex biological process involving the coordination of multiple hormonal signaling pathways, transcription factors, and environmental cues. Strigolactones (SLs), a recently identified class of plant hormones, play essential roles in suppressing branching, modulating root architecture, and mediating drought stress responses. Our preliminary observations revealed that the SL signaling suppressor mutant s678 exhibits markedly altered branching phenotypes compared to wild-type Arabidopsis. Additionally, the cryptochrome CRY1 mutant displays increased rosette leaf branching, yet whether CRY1 participates in SL-mediated regulation of Arabidopsis growth and development remains unclear. In this study, we employed bimolecular fluorescence complementation (BiFC), yeast two-hybrid (Y2H), and luciferase complementation assays to demonstrate that CRY1 interacts with SMXL7 both in vivo and in vitro. Furthermore, we discovered that CRY1 interacts with SMXL6/7/8, key SL signaling repressors, and modulates Arabidopsis branching. These findings provide novel insights into the molecular mechanisms underlying SL-regulated branching and establish a foundational framework for understanding the crosstalk between light signaling (mediated by CRY1) and SL signaling in plant development. This work also offers theoretical support for optimizing plant architecture under varying light conditions, with potential applications in crop improvement.