PRMT3介导的甲基化修饰在胚胎干细胞分化过程中的作用及机制研究

rginine methylation is a common post-translational modification of proteins, catalyzed by the Protein arginine methyltransferases (PRMTs) family of proteins. PRMTs regulate a variety of biological processes such as cell differentiation, embryonic development, and glycolipid metabolism, through methylation of their cytoplasmic or nucleus-specific substrates. biological processes. As a unique PRMT, PRTM3 is widely expressed in various cell types, but the biological function of PRMT3 in regulating differentiation processes in Embryonic Stem Cells (ESCs) remains unknown. In this study, we used the differentiation of mouse ESCs (mESCs) into Embryoid Body (EB) as a research model to investigate the role of PRMT3 in the differentiation process of ESCs and the specific molecular mechanism. The results showed that inhibition of PRMT3 during the induction of ESCs differentiation into EBs would specifically down-regulate the expression levels of the neural ectoderm-related marker proteins NESTIN, SOX2, and PAX6, as well as decrease the expression levels of the enzymes related to glycolipid metabolism FASN, SCD1, and PKM1/2. In contrast, overexpression of PRMT3 upregulated the expression levels of NESTIN, FASN, and SCD1. Taken together, the results of the present study imply that PRMT3 may affect the differentiation process of ESCs to neural ectoderm through the regulation of glycolipid metabolism, and then affect the differentiation process of ESCs to neural ectoderm.