牦牛骨源抗炎肽改善皮肤炎症的作用与机制

Skin inflammation can contribute to various skin disorders. Anti-inflammatory peptides have been widely used in treating skin conditions due to their strong therapeutic effects and minimal side effects. In our previous study, six anti-inflammatory peptides were identified from yak bone collagen, though their effects on skin inflammation remained unexplored. To further investigate the potential applications of these peptides, we examined the anti-inflammatory effects and mechanisms of the selected peptide, GPAGPSGPAGKDGR (Seq2), using a lipopolysaccharide (LPS)-induced HaCaT cell inflammation model. Additionally, we assessed the peptide\'s stability under various environmental conditions to evaluate its practical applicability.At a concentration of 100 μM, Seq2 significantly inhibited the production of key inflammatory mediators, with suppression rates of 85.71 ± 1.40% for IL-6, 78.10 ± 4.02% for IL-1β, 80.60 ± 1.34% for TNF-α, and 94.79 ± 0.48% for NO. Furthermore, Seq2 treatment reduced the p-P65/t-P65 and p-IκB/t-IκB ratios in HaCaT cells by approximately 32.11% and 39.39%, respectively, compared to the model group, suggesting modulation of the NF-κB signaling pathway.Stability tests revealed that Seq2 remained highly stable at temperatures below 50 C, and its structure was largely unaffected by pH variations, light exposure, metal ions, or common processing aids, retaining around 90% of its initial concentration under these conditions.These findings demonstrate that Seq2, a yak bone collagen-derived peptide, exhibits excellent stability and effectively alleviates inflammation in HaCaT cells by regulating the NF-κB pathway. This study expands the potential applications of food-derived anti-inflammatory peptides and provides new insights for future research on yak bone proteins.