乳酸代谢在骨骼肌能量稳态及运动调控中的作用研究

Energy metabolism homeostasis is fundamental to maintaining normal physiological functions. With the growth of the global population and the intensification of aging, metabolic disorders caused by aging have become a significant public health challenge. Exercise intervention has been shown to effectively mitigate muscle degeneration during aging. During exercise, lactate released into circulation through glycolysis in skeletal muscle cells not only serves as an energy substrate but may also function as a signaling molecule involved in regulating gene expression and protein levels in various cell types. In this study, based on multi-saThe Role of Lactate Metabolism in Skeletal Muscle Energy Homeostasis and Exercise Regulationmple gene expression sequencing of the deltoid skeletal muscle tissue in humans, we found that the glycolytic pathway in skeletal muscle undergoes changes in the elderly population. Notably, the levels of lactate dehydrogenase isoforms LDHA and LDHB exhibited significant alterations, suggesting that lactate may play a crucial role in skeletal muscle aging. To further investigate this, we conducted exercise performance assessments in skeletal muscle-specific Ldhb knockout (LdhbMKO) mice to examine the impact of lactate on skeletal muscle function. Our results indicate that when the glycolytic pathway in skeletal muscle is activated during exercise, impaired lactate metabolism (LdhbMKO) leads to increased oxygen consumption in mice. Additionally, ATGL protein and lactylated proteins were upregulated, thereby activating lipid metabolism pathways to provide an alternative energy source for skeletal muscle. These findings provide potential insights into the role of lactate metabolism in maintaining skeletal muscle function and offer implications for strategies aimed at enhancing exercise performance.