硫酸软骨素蛋白聚糖类似物微球的制备与表征

In order to simulate the comb-like structure of natural chondroitin sulfate proteoglycan in extracellular matrix, this study first synthesized methacryloyl gelatin with low grafting degree, and rationally designed a chondroitin sulfate proteoglycan analog: low molecular weight chondroitin sulfate-methacryloyl gelatin (GMCS) with it as the core protein. In this paper, the effects of grafting chondroitin sulfate with different molecular weights on the structure and physical properties of methacrylated gelatin were studied by Fourier infrared spectroscopy, Zeta potential analyzer and rheometer. GMCS were screened by cytotoxicity test and successfully used to prepare microspheres supporting nerve regeneration. The results show that the grafting of chondroitin sulfate can improve the hydrophilicity and electronegativity of methacrylated gelatin. When the molecular weight of chondroitin sulfate grafted by GMCS is 1 ~ 3 kDa, GMCS can retain the photocurable characteristics of the main chain of methacrylated gelatin and promote the growth of neurons. GMCS hydrogel microspheres prepared by microfluidic technology have uniform size and smooth surface, and have broad application prospects in tissue engineering.