玉米细胞壁cellulose synthase基因家族的细胞特异性表达、调控的研究

ellulose Synthase (CesA) plays a vital role in the biosyntheCell-specific expression and regulation of cellulose synthase gene family in maize cell wallsis of plant cell walls and is a key enzyme in the cellulose biosynthesis pathway. Cellulose is one of the main components of plant cell wall and provides stable support structure for plant body. Therefore, cellulose synthase plays an important role in plant growth and development and plant defense response. The development of C4 rice faces many challenges, including how to optimize its cell wall structure to improve stress resistance and yield, and corn is a typical C4 crop, so the study of cellulose synthase is of reference significance for the development of C4 rice. At present, the regulation of specific high expression of CesA gene family in maize cell wall in BS has not been found. Therefore, in this paper, a total of 43 CesA genes were found in the corn gene bank, and the members of the CesA gene family were constructed evolutionary tree, made gene expression heat map and string database analysis. It was found that the CesA gene family was specifically expressed in corn tissues and cells, and had a regulatory effect on the growth and development of corn. miRNA cleavage was performed on the highly expressed gene (GRMZM2G012044) in the vascular bundle sheath cells (BS), and corn genes in a miRNA regulatory network jointly affected the C4 photosynthetic metabolism process by affecting the light response, signal transduction process and metabolic reaction process in the C4 vascular bundle sheath cells, respectively. In this paper, the specific expression of CesA gene family in BS was studied to provide theoretical basis for cellulose synthesis mechanism and the anatomical structure of C4 garland.