MLP调控MDVs介导肺癌放疗后炎症

OBJECTIVE: Pneumonia is induced after radiotherapy for lung cancer is a common complication of radiotherapy, but the mechanism remains unclear and needs to be studied urgently. METHODS: ATMLP overexpression and knockdown cell lines were constructed by gene editing techniques, and Western Blot, immunofluorescence, and Transwell co-culture were used to investigate the role of MDVs in inflammation induced by radiotherapy for pneumonia, and the effects of ATMLP, DRP-1, and PGAM5 on MDVs. RESULTS: Ionizing radiation promoted the expression of ATMLP as well as MDVs. Increased expression of ATMLP led to up-regulation of PGAM5 expression as well as pDRP-1 (ser616).Mechanistic of ATMLP Regulation of Mitochondrial-Derived Vesicle Mediated Radiotherapy Induced Pulmonary Inflammation in Lung CancerIncreased number of MDVs led to increased levels of macrophage M1-type polarization. CONCLUSION: Ionizing radiation can lead to an increase in the cellular ATMLP expression level, and the increase in ATMLP can contribute to the increase in the number of MDVs through the PGAM5/DRP-1 axis, leading to M1-type polarization of macrophages and promoting inflammation.