光甘草酮对皮肤黑色素合成的抑制作用及其机理研究

Objective: To elucidate the inhibitory effect of Glabrone on melanogenesis and its underlying mechanisms. Methods: The inhibitory effects of Glabrone on melanin synthesis was evaluated by the B16 cell model. Subsequently, to understand the mechanism, the tyrosinase activity on B16 cell and enzyme assay were used. Next, the antioxidant capacity of Glabrone was assessed by the DPPH scavenging assay and the UVB induced intracellular oxygen species (ROS) scavenging assay. To explore the physiological process of Glabrone, qRT-PCR was used to evaluate the mRNA expression of tyrosinase (TYR), microphthalmia-associated transcription factor (MITF), and cAMP response element-binding protein (CREB) on B16 cells. Finally, the melanogenesis inhibitory effect of Glabrone on C57BL/6J mice induced by UVB irradiation was declared. Results: Glabrone significantly reduced the melanin synthesis in B16 cells, inhibited both intracellular and extracellular tyrosinase activities, with inhibition rates of 29.7%, and 30%, respectively. Compared with Glabridin, Glabrone show a better effect on DPPH free radicals scavenging, intracellular ROS cleaning, and downregulating MITF, TYR, and CREB gene expression. The animal experiments confirmed that Glabrone effectively reduce UVB-induced skin pigmentation. Conclusion: Glabrone inhibits melanogenesis by suppressing the expression and activity of tyrosinase primarily. The mechanism of Glabrone may involves regulating oxidative stress-related pathways, modulating the PKC-PKA-CREB signaling cascade and downregulating of MITF and TYR gene expression..