RNA结合蛋白AKAP95基因组结合位点的鉴定

-kinase anchoring protein 95 (AKAP95), localized in the nucleus, is an RNA-binding protein. Previous studies have found that AKAP95 participates in transcriptional regulation as a transcriptional cofactor. However, little is known about the details. In this study, we successfully constructed an endogenous expression of Flag-mEGFP-AKAP95 Hela cell line by CRISPR-Cas9 technology. We performed Flag ChIP-seq experiments on it to explore AKAP95 interaction with chromatin, and found that AKAP95 widely bound to genomic DNA, especially at the gene promoters. Motif analysis revealed that the binding promoters of AKAP95 were enriched with four transcription factors (NFY, Sp1, YY1, Elk4) binding motifs, suggesting AKAP95 may be involved in transcription activation through binding to genomic DNA. The present study lays the foundation for further exploration of the biophysical mechanism of AKAP95 involved in transcription activation.